Cdc53p acts in concert with cdc4p and cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins

Neal Mathias, Stephen L. Johnson, Mark Winey, Alison E M Adams, Loretta Goetsch, John R. Pringle, Breck Byers, Mark Goebl

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin- dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.

Original languageEnglish
Pages (from-to)6634-6643
Number of pages10
JournalMolecular and Cellular Biology
Volume16
Issue number12
StatePublished - 1996

Fingerprint

Phase Transition
S Phase
Cell Cycle
Proteins
Ubiquitin-Conjugating Enzymes
Mutation
Cyclin-Dependent Kinases
Proteolysis
Yeasts
Cell Proliferation
Phenotype

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cdc53p acts in concert with cdc4p and cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins. / Mathias, Neal; Johnson, Stephen L.; Winey, Mark; Adams, Alison E M; Goetsch, Loretta; Pringle, John R.; Byers, Breck; Goebl, Mark.

In: Molecular and Cellular Biology, Vol. 16, No. 12, 1996, p. 6634-6643.

Research output: Contribution to journalArticle

Mathias, N, Johnson, SL, Winey, M, Adams, AEM, Goetsch, L, Pringle, JR, Byers, B & Goebl, M 1996, 'Cdc53p acts in concert with cdc4p and cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins', Molecular and Cellular Biology, vol. 16, no. 12, pp. 6634-6643.
Mathias, Neal ; Johnson, Stephen L. ; Winey, Mark ; Adams, Alison E M ; Goetsch, Loretta ; Pringle, John R. ; Byers, Breck ; Goebl, Mark. / Cdc53p acts in concert with cdc4p and cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins. In: Molecular and Cellular Biology. 1996 ; Vol. 16, No. 12. pp. 6634-6643.
@article{ec1e778056ed465ebf380c7fa852fa4a,
title = "Cdc53p acts in concert with cdc4p and cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins",
abstract = "Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin- dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.",
author = "Neal Mathias and Johnson, {Stephen L.} and Mark Winey and Adams, {Alison E M} and Loretta Goetsch and Pringle, {John R.} and Breck Byers and Mark Goebl",
year = "1996",
language = "English",
volume = "16",
pages = "6634--6643",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Cdc53p acts in concert with cdc4p and cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins

AU - Mathias, Neal

AU - Johnson, Stephen L.

AU - Winey, Mark

AU - Adams, Alison E M

AU - Goetsch, Loretta

AU - Pringle, John R.

AU - Byers, Breck

AU - Goebl, Mark

PY - 1996

Y1 - 1996

N2 - Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin- dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.

AB - Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin- dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.

UR - http://www.scopus.com/inward/record.url?scp=0029860817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029860817&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 6634

EP - 6643

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 12

ER -