cDNA approaches to isolation of the mouse mutant weaver gene

M. E. Hodes, S. R. Dlouhy, J. J. Wei, Y. Wang, L. Sangameswaran, V. Lazar, L. C. Triarhou, B. Ghetti

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.

Original languageEnglish (US)
Pages (from-to)1359-1362
Number of pages4
JournalNeurochemical Research
Volume19
Issue number11
DOIs
StatePublished - Nov 1 1994

    Fingerprint

Keywords

  • granule cells
  • mutant mice
  • sequence, in situ hybridization
  • Weaver

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Hodes, M. E., Dlouhy, S. R., Wei, J. J., Wang, Y., Sangameswaran, L., Lazar, V., Triarhou, L. C., & Ghetti, B. (1994). cDNA approaches to isolation of the mouse mutant weaver gene. Neurochemical Research, 19(11), 1359-1362. https://doi.org/10.1007/BF00972463