cDNA approaches to isolation of the mouse mutant weaver gene

M. E. Hodes, Stephen Dlouhy, J. J. Wei, Y. Wang, L. Sangameswaran, V. Lazar, L. C. Triarhou, Bernardino Ghetti

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.

Original languageEnglish
Pages (from-to)1359-1362
Number of pages4
JournalNeurochemical Research
Volume19
Issue number11
DOIs
StatePublished - Nov 1994

Fingerprint

Neurologic Mutant Mice
Gene Library
Complementary DNA
Genes
Recessive Genes
Nucleic Acid Repetitive Sequences
Substantia Nigra
Cerebellum
Libraries
Testis
Screening
Clone Cells

Keywords

  • granule cells
  • mutant mice
  • sequence, in situ hybridization
  • Weaver

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Hodes, M. E., Dlouhy, S., Wei, J. J., Wang, Y., Sangameswaran, L., Lazar, V., ... Ghetti, B. (1994). cDNA approaches to isolation of the mouse mutant weaver gene. Neurochemical Research, 19(11), 1359-1362. https://doi.org/10.1007/BF00972463

cDNA approaches to isolation of the mouse mutant weaver gene. / Hodes, M. E.; Dlouhy, Stephen; Wei, J. J.; Wang, Y.; Sangameswaran, L.; Lazar, V.; Triarhou, L. C.; Ghetti, Bernardino.

In: Neurochemical Research, Vol. 19, No. 11, 11.1994, p. 1359-1362.

Research output: Contribution to journalArticle

Hodes, ME, Dlouhy, S, Wei, JJ, Wang, Y, Sangameswaran, L, Lazar, V, Triarhou, LC & Ghetti, B 1994, 'cDNA approaches to isolation of the mouse mutant weaver gene', Neurochemical Research, vol. 19, no. 11, pp. 1359-1362. https://doi.org/10.1007/BF00972463
Hodes ME, Dlouhy S, Wei JJ, Wang Y, Sangameswaran L, Lazar V et al. cDNA approaches to isolation of the mouse mutant weaver gene. Neurochemical Research. 1994 Nov;19(11):1359-1362. https://doi.org/10.1007/BF00972463
Hodes, M. E. ; Dlouhy, Stephen ; Wei, J. J. ; Wang, Y. ; Sangameswaran, L. ; Lazar, V. ; Triarhou, L. C. ; Ghetti, Bernardino. / cDNA approaches to isolation of the mouse mutant weaver gene. In: Neurochemical Research. 1994 ; Vol. 19, No. 11. pp. 1359-1362.
@article{d9d28ab8e33d4a2fbe2e57641d400612,
title = "cDNA approaches to isolation of the mouse mutant weaver gene",
abstract = "The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.",
keywords = "granule cells, mutant mice, sequence, in situ hybridization, Weaver",
author = "Hodes, {M. E.} and Stephen Dlouhy and Wei, {J. J.} and Y. Wang and L. Sangameswaran and V. Lazar and Triarhou, {L. C.} and Bernardino Ghetti",
year = "1994",
month = "11",
doi = "10.1007/BF00972463",
language = "English",
volume = "19",
pages = "1359--1362",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "11",

}

TY - JOUR

T1 - cDNA approaches to isolation of the mouse mutant weaver gene

AU - Hodes, M. E.

AU - Dlouhy, Stephen

AU - Wei, J. J.

AU - Wang, Y.

AU - Sangameswaran, L.

AU - Lazar, V.

AU - Triarhou, L. C.

AU - Ghetti, Bernardino

PY - 1994/11

Y1 - 1994/11

N2 - The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.

AB - The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.

KW - granule cells

KW - mutant mice

KW - sequence, in situ hybridization

KW - Weaver

UR - http://www.scopus.com/inward/record.url?scp=0027948957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027948957&partnerID=8YFLogxK

U2 - 10.1007/BF00972463

DO - 10.1007/BF00972463

M3 - Article

C2 - 7898606

AN - SCOPUS:0027948957

VL - 19

SP - 1359

EP - 1362

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 11

ER -