Celecoxib inhibits ureteral contractility and prostanoid release

Travis J. Jerde, Jamie L. Calamon-Dixon, Dale E. Bjorling, Stephen Y. Nakada

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

To evaluate the efficacy and potency of clinically available celecoxib for inhibition of ureteral contractility and prostanoid release. We have previously reported that the selective cyclooxygenase (COX)-2 inhibitor NS-398 inhibits ureteral contractility. We evaluated the release of prostaglandin (PG) E 2, F, D2, thromboxane B2 (a thromboxane2 metabolite), and 6-keto-PGF (a prostacyclin metabolite) by gas chromatography-mass spectrometry from porcine ureters in the presence and absence of tumor necrosis factor-alpha (TNF-α), a putative cyclooxygenase (COX)-2 inducer. PGE2 and PGF were the prostanoids released in greatest quantity in response to TNF-α. We subsequently measured spontaneous contractility and prostanoid release in porcine ureters treated with 0.1, 1.0, or 10 μM concentrations of indomethacin (nonselective COX inhibitor), NS-398, celecoxib, or 0.1% dimethyl sulfoxide (vehicle) for 2 hours. Ureteral contractility and prostanoid release were measured every 15 minutes after the addition of the various compounds. We also treated ureters with 10 ng/mL TNF-α and all three COX inhibitors or dimethyl sulfoxide for 2 and 4 hours and measured the PGE2 and PGF release. Celecoxib, indomethacin, and NS-398 inhibited ureteral contractility and prostanoid release with similar efficacy and potency. All three compounds also reduced TNF-α-induced prostanoid release to control levels at concentrations as low as 0.1 μM. Our data have indicated that celecoxib and indomethacin inhibit PG release by the ureter to a similar degree, even in the presence of COX-2 induction. Animal experiments and clinical trials evaluating the safety and efficacy of celecoxib for the treatment of symptomatic ureteral obstruction are warranted.

Original languageEnglish (US)
Pages (from-to)185-190
Number of pages6
JournalUrology
Volume65
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Urology

Fingerprint Dive into the research topics of 'Celecoxib inhibits ureteral contractility and prostanoid release'. Together they form a unique fingerprint.

  • Cite this