Cell cycle kinetics of permanent glioma cells measured by pulse labelling with bromodeoxyuridine

F. Fogt, A. O. Vortmeyer, S. R. Tahan, D. Stavrou, N. W. Istfan

Research output: Contribution to journalArticle

Abstract

Cell phase distribution and cycle kinetics of six human glioblastoma cell lines were characterized after labelling with 5-Bromo-2-deoxyuridine (BrdUrd). Cycle time (T(c)), DNA synthesis time (T(s)), and potential doubling time (T(pot)) were compared with the actual doubling time (T(d)) of the growing cell population. Mathematical estimates closely correlated with T(d). Low labelling index (LI) correlated with short T(s) and vice versa. T(s) and LI allowed grouping of the cell lines in two clusters. The mean number of silver stained nucleolar organizer regions (mAgNORs) and percentage of cells with more than five AgNORs (pAgNOR) were counted. AgNORs closely related to LI. Low mAgNORs and pAgNORs correlated with fast T(s) among the clustered cell lines.

Original languageEnglish (US)
Pages (from-to)1315-1321
Number of pages7
JournalInternational Journal of Oncology
Volume4
Issue number6
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • cell cycle kinetics
  • glioma
  • nuclear organizer regions

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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