Cell cycle measurement of mouse hematopoietic stem/progenitor cells

Brahmananda Reddy Chitteti, Edward F. Srour

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Lifelong production of blood cells is sustained by hematopoietic stem cells (HSC). HSC reside in a mitotically quiescent state within specialized areas of the bone marrow (BM) microenvironment known as the hematopoietic niche (HN). HSC enter into active phases of cell cycle in response to intrinsic and extrinsic biological cues thereby undergoing differentiation or self-renewal divisions. Quiescent and mitotically active HSC have different metabolic states and different functional abilities such as engraftment and BM repopulating potential following their transplantation into conditioned recipients. Recent studies reveal that various cancers also utilize the same mechanisms of quiescence as normal stem cells and preserve the root of malignancy thus contributing to relapse and metastasis. Therefore, exploring the stem cell behavior and function in conjunction with their cell cycle status has significant clinical implications in HSC transplantation and in treating cancers. In this chapter, we describe methodologies to isolate or analytically measure the frequencies of quiescent (G0) and active (G1, S, and G2-M) hematopoietic progenitor and stem cells among murine BM cells.

Original languageEnglish (US)
Title of host publicationHematopoietic Stem Cell Protocols
PublisherHumana Press Inc.
Pages65-78
Number of pages14
ISBN (Print)9781493911325
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1185
ISSN (Print)1064-3745

Keywords

  • CD71
  • Cell cycle
  • Hematopoietic stem cells
  • Hoechst 33342
  • Progenitor cells
  • Pyronin Y

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    Chitteti, B. R., & Srour, E. F. (2014). Cell cycle measurement of mouse hematopoietic stem/progenitor cells. In Hematopoietic Stem Cell Protocols (pp. 65-78). (Methods in Molecular Biology; Vol. 1185). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-1133-2_5