Cell density influences hormonal responsiveness but not lipoprotein utilization in cultured bovine luteal cells

J. L. Pate, K. P. Nephew, G. S. Zarle

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The objective of the present study was to investigate the effect of cell density on hormonal responsiveness and lipoprotein utilization by cultured bovine luteal cells. Luteal cells obtained from regularly cycling dairy cows were plated at three culture densities: 0.5 × 106, 1 × 106 and 2 × 106 cells/flask in serum-free Ham's F-12 culture medium, and maintained for 9 days. Basal steroidogenesis was unaffected by cell density, while LH responsiveness was greatest in low density cultures. Progesterone produced in response to LH (10 ng/ml) was greater than control levels throughout the culture period in low density cultures. Luteal cells cultured at medium and high densities became responsive to LH only later in the culture period (days 5 and 9, respectively). In contrast, prostaglandin F (PGF, 100 ng/ml) was more effective in high density cultures, causing a complete inhibition of LH stimulation and returning progesterone levels to basal values. In low density cultures, treatment with PGF + LH resulted in progesterone levels that were not significantly different from LH-treated cultures. There was no effect of cell density on utilization of either low density lipoprotein (LDL) or high density lipoprotein (HDL) for steroidogenesis. However, a synergistic effect of LH with either lipoprotein was observed in low and medium density, but not high density cultures. From these results, it is concluded that culture density can influence the responsiveness of bovine luteal cells to either LH or PGF, but has no effect on lipoprotein utilization by these cells.

Original languageEnglish (US)
Pages (from-to)187-194
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume53
Issue number3
DOIs
StatePublished - Oct 1987
Externally publishedYes

Keywords

  • Cell culture
  • Cell density
  • Corpus luteum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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