Abstract
Human polymorphonuclears (PMN) or granulocytes (nonadherent: density > 1.070 g/cm3) were used to prepare a cell free colony inhibiting activity (CIA). Intact PMN, PMN conditioned media (serum free or serum containing) and supernatant from immediately lysed PMN equally reduced the numbers of spontaneous bone marrow (density <1.070 g/cm3; depleted of PMN colonies and clusters. Addition of CIA reduced reproducibly colony and/or cluster numbers by 35 to 70%. CIA was heat labile being inactivated at 37°C. Daily additions of CIA reduced colony numbers even further; inhibition was dependent on [PMN] used to prepare CIA and on the potency of stimulation produced by colony stimulating cells (CSC). PMN from some patients with leukemia were inactive as inhibitors and did not condition media with CIA. All experiments were thereafter controlled by parallel assessment of supernatant of lysed PMN not demonstrating any CIA. CIA appears to be not only stored in PMN but released from viable, as well as degranulating PMN. Although not species specific, CIA was specific in its nontoxic action on CSC; colony forming cells and cell free colony stimulating activity were not affected. The influence of CIA on CSC could be reversed within 10 min, but not within 35 min of incubation. CIA may have possible physiologic relevance.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 87-102 |
| Number of pages | 16 |
| Journal | Experimental Hematology |
| Volume | 5 |
| Issue number | 2 |
| State | Published - 1977 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Cell Biology
- Genetics
- Hematology
- Oncology
- Transplantation
Cite this
Cell free granulocyte colony inhibiting activity derived from human polymorphonuclear neutrophils. / Broxmeyer, Hal; Moore, M. A S; Ralph, P.
In: Experimental Hematology, Vol. 5, No. 2, 1977, p. 87-102.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell free granulocyte colony inhibiting activity derived from human polymorphonuclear neutrophils
AU - Broxmeyer, Hal
AU - Moore, M. A S
AU - Ralph, P.
PY - 1977
Y1 - 1977
N2 - Human polymorphonuclears (PMN) or granulocytes (nonadherent: density > 1.070 g/cm3) were used to prepare a cell free colony inhibiting activity (CIA). Intact PMN, PMN conditioned media (serum free or serum containing) and supernatant from immediately lysed PMN equally reduced the numbers of spontaneous bone marrow (density <1.070 g/cm3; depleted of PMN colonies and clusters. Addition of CIA reduced reproducibly colony and/or cluster numbers by 35 to 70%. CIA was heat labile being inactivated at 37°C. Daily additions of CIA reduced colony numbers even further; inhibition was dependent on [PMN] used to prepare CIA and on the potency of stimulation produced by colony stimulating cells (CSC). PMN from some patients with leukemia were inactive as inhibitors and did not condition media with CIA. All experiments were thereafter controlled by parallel assessment of supernatant of lysed PMN not demonstrating any CIA. CIA appears to be not only stored in PMN but released from viable, as well as degranulating PMN. Although not species specific, CIA was specific in its nontoxic action on CSC; colony forming cells and cell free colony stimulating activity were not affected. The influence of CIA on CSC could be reversed within 10 min, but not within 35 min of incubation. CIA may have possible physiologic relevance.
AB - Human polymorphonuclears (PMN) or granulocytes (nonadherent: density > 1.070 g/cm3) were used to prepare a cell free colony inhibiting activity (CIA). Intact PMN, PMN conditioned media (serum free or serum containing) and supernatant from immediately lysed PMN equally reduced the numbers of spontaneous bone marrow (density <1.070 g/cm3; depleted of PMN colonies and clusters. Addition of CIA reduced reproducibly colony and/or cluster numbers by 35 to 70%. CIA was heat labile being inactivated at 37°C. Daily additions of CIA reduced colony numbers even further; inhibition was dependent on [PMN] used to prepare CIA and on the potency of stimulation produced by colony stimulating cells (CSC). PMN from some patients with leukemia were inactive as inhibitors and did not condition media with CIA. All experiments were thereafter controlled by parallel assessment of supernatant of lysed PMN not demonstrating any CIA. CIA appears to be not only stored in PMN but released from viable, as well as degranulating PMN. Although not species specific, CIA was specific in its nontoxic action on CSC; colony forming cells and cell free colony stimulating activity were not affected. The influence of CIA on CSC could be reversed within 10 min, but not within 35 min of incubation. CIA may have possible physiologic relevance.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017357932&partnerID=8YFLogxK
M3 - Article
C2 - 265274
AN - SCOPUS:0017357932
VL - 5
SP - 87
EP - 102
JO - Experimental Hematology
JF - Experimental Hematology
SN - 0301-472X
IS - 2
ER -