Cell proliferation and migration are modulated by Cdk-1-phosphorylated Endothelial-Monocyte Activating Polypeptide II

Margaret Schwarz, Janet Thornton, Haiming Xu, Niranjan Awasthi, Roderich E. Schwarz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Endothelial-Monocyte Activating Polypeptide (EMAP II) is a secreted protein with well-established anti-angiogenic activities. Intracellular EMAP II expression is increased during fetal development at epithelial/mesenchymal boundaries and in pathophysiologic fibroproliferative cells of bronchopulmonary dysplasia, emphysema, and scar fibroblast tissue following myocardial ischemia. Precise function and regulation of intracellular EMAP II, however, has not been explored to date. Methodology/Principal Findings: Here we show that high intracellular EMAP II suppresses cellular proliferation by slowing progression through the G2M cell cycle transition in epithelium and fibroblast. Furthermore, EMAP II binds to and is phosphorylated by Cdk1, and exhibits nuclear/cytoplasmic partitioning, with only nuclear EMAP II being phosphorylated. We observed that extracellular secreted EMAP II induces endothelial cell apoptosis, where as excess intracellular EMAP II facilitates epithelial and fibroblast cells migration. Conclusions/Significance: Our findings suggest that EMAP II has specific intracellular effects, and that this intracellular function appears to antagonize its extracellular anti-angiogenic effects during fetal development and pulmonary disease progression.

Original languageEnglish (US)
Article numbere33101
JournalPLoS One
Volume7
Issue number3
DOIs
StatePublished - Mar 8 2012
Externally publishedYes

Fingerprint

Cell proliferation
cell movement
monocytes
Cell Movement
fibroblasts
polypeptides
cell proliferation
Cell Proliferation
fetal development
bronchopulmonary dysplasia
Fibroblasts
myocardial ischemia
disease course
respiratory tract diseases
endothelial cells
cell cycle
epithelial cells
epithelium
apoptosis
Fetal Development

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cell proliferation and migration are modulated by Cdk-1-phosphorylated Endothelial-Monocyte Activating Polypeptide II. / Schwarz, Margaret; Thornton, Janet; Xu, Haiming; Awasthi, Niranjan; Schwarz, Roderich E.

In: PLoS One, Vol. 7, No. 3, e33101, 08.03.2012.

Research output: Contribution to journalArticle

Schwarz, Margaret ; Thornton, Janet ; Xu, Haiming ; Awasthi, Niranjan ; Schwarz, Roderich E. / Cell proliferation and migration are modulated by Cdk-1-phosphorylated Endothelial-Monocyte Activating Polypeptide II. In: PLoS One. 2012 ; Vol. 7, No. 3.
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