Cell surface association of matrix metalloproteinase-9 (gelatinase B)

Rafael Fridman, Marta Toth, Irina Chvyrkova, Samy Meroueh, Shahriar Mobashery

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Matrix metalloproteinase (MMP)-9 (gelatinase B) belongs to the MMP family of zinc-dependent endopeptidases that has been associated with tumor cell invasion and metastasis and tumor-induced angiogenesis. As a secreted MMP, pro-MMP-9 is released into the extracellular environment by both tumor and stroma cells, where it fulfills its proteolytic functions degrading both extracellular matrix (ECM) and non-ECM proteins. A major dilemma in our understanding of MMP-9 function is how the released protease is targeted to the right location and how its activity is controlled at the pericellular space. It has been proposed that MMP-9 interact with cell surface components and that this type of interaction positively regulates enzymatic activation and activity. However, recent evidence shows that association of MMP-9 with the cell surface is mediated by a distinct array of surface proteins that serve to regulate multiple aspects of the enzyme function including localization, inhibition and internalization. How these distinct mechanisms regulate the overall MMP-9 activity at the pericellular space remains an important goal in our understanding of MMP-9 function at the cell surface. Furthermore, the study of surface-associated MMP-9 imposes new conceptual and methodological challenges with particular consideration to the unique structural and functional characteristics of this key enzyme.

Original languageEnglish (US)
Pages (from-to)153-166
Number of pages14
JournalCancer and Metastasis Reviews
Volume22
Issue number2-3
DOIs
StatePublished - Jun 2003
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 9
Secreted Matrix Metalloproteinases
Neoplasms
Endopeptidases
Enzymes
Cellular Structures
Matrix Metalloproteinases
Extracellular Matrix
Zinc
Peptide Hydrolases
Neoplasm Metastasis

Keywords

  • Cell surface binding
  • Matrix metalloproteinase
  • Protease
  • Zymogen

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Cell surface association of matrix metalloproteinase-9 (gelatinase B). / Fridman, Rafael; Toth, Marta; Chvyrkova, Irina; Meroueh, Samy; Mobashery, Shahriar.

In: Cancer and Metastasis Reviews, Vol. 22, No. 2-3, 06.2003, p. 153-166.

Research output: Contribution to journalArticle

Fridman, Rafael ; Toth, Marta ; Chvyrkova, Irina ; Meroueh, Samy ; Mobashery, Shahriar. / Cell surface association of matrix metalloproteinase-9 (gelatinase B). In: Cancer and Metastasis Reviews. 2003 ; Vol. 22, No. 2-3. pp. 153-166.
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