Cell surface receptors for herpes simplex virus are heparan sulfate proteoglycans

M. T. Shieh, D. WuDunn, R. I. Montgomery, J. D. Esko, P. G. Spear

Research output: Contribution to journalArticle

421 Scopus citations

Abstract

The role of cell surface heparan sulfate in herpes simplex virus (HSV) infection was investigated using CHO cell mutants defective in various aspects of glycosaminoglycan synthesis. Binding of radiolabeled virus to the cells and infection were assessed in mutant and wild-type cells. Virus bound efficiently to wild-type cells and initiated an abortive infection in which immediate-early or α viral genes were expressed, despite limited production of late viral proteins and progeny virus. Binding of virus to heparan sulfate-deficient mutant cells was severely impaired and mutant cells were resistant to HSV infection. Intermediate levels of binding and infection were observed for a CHO cell mutant that produced undersulfated heparan sulfate. These results show that heparan sulfate moieties of cell surface proteoglycans serve as receptors for HSV.

Original languageEnglish (US)
Pages (from-to)1273-1281
Number of pages9
JournalJournal of Cell Biology
Volume116
Issue number5
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Cell Biology

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    Shieh, M. T., WuDunn, D., Montgomery, R. I., Esko, J. D., & Spear, P. G. (1992). Cell surface receptors for herpes simplex virus are heparan sulfate proteoglycans. Journal of Cell Biology, 116(5), 1273-1281. https://doi.org/10.1083/jcb.116.5.1273