Cell wall glycosphingolipids of Sphingomonas paucimobilis are CD1d-specific ligands for NKT cells

Venkataraman Sriram, Wenjun Du, Jacquelyn Gervay-Hague, Randy Brutkiewicz

Research output: Contribution to journalArticle

249 Citations (Scopus)

Abstract

The current consensus on characterization of NKT cells is based on their reactivity to the synthetic glycolipid, α-galactosylceramide (α-GalCer) in a CD1d-dependent manner. Because of the limited availability of α-GalCer, there is a constant search for CD1d-presented ligands that activate NKT cells. The α-anomericity of the carbohydrate is considered to be an important requisite for the CD1d-specific activation of NKT cells. The gram-negative, lipopolysaccharide-free bacterium Sphingomonas paucimobilis is known to contain glycosphingolipids (GSL) with α-anomeric sugars attached to the lipid chain. Here, we report that GSL extracted from this bacterium are able to stimulate NKT cells in a CD1d-specific manner. In addition, soluble CD1d-Ig dimers loaded with this lipid extract specifically bind to NKT cells (but not conventional T cells). Further studies on the S. paucimobilis GSL could potentially lead to other natural sources of CD1d-specific ligands useful for NKT cell analyses and aimed at identifying novel therapies for a variety of disease states.

Original languageEnglish
Pages (from-to)1692-1701
Number of pages10
JournalEuropean Journal of Immunology
Volume35
Issue number6
DOIs
StatePublished - Jun 2005

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Sphingomonas
Glycosphingolipids
Natural Killer T-Cells
Cell Wall
Ligands
Galactosylceramides
Bacteria
Lipids
Glycolipids
Lipopolysaccharides
Carbohydrates
T-Lymphocytes

Keywords

  • Antigen presentation
  • Glycosphingolipids
  • NKT cell activation

ASJC Scopus subject areas

  • Immunology

Cite this

Cell wall glycosphingolipids of Sphingomonas paucimobilis are CD1d-specific ligands for NKT cells. / Sriram, Venkataraman; Du, Wenjun; Gervay-Hague, Jacquelyn; Brutkiewicz, Randy.

In: European Journal of Immunology, Vol. 35, No. 6, 06.2005, p. 1692-1701.

Research output: Contribution to journalArticle

Sriram, Venkataraman ; Du, Wenjun ; Gervay-Hague, Jacquelyn ; Brutkiewicz, Randy. / Cell wall glycosphingolipids of Sphingomonas paucimobilis are CD1d-specific ligands for NKT cells. In: European Journal of Immunology. 2005 ; Vol. 35, No. 6. pp. 1692-1701.
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