Cells of cutaneous immunity in Xenopus

Studies during larval development and limb regeneration

Anthony Mescher, Wendy L. Wolf, E. Ashley Moseman, Byron Hartman, Clint Harrison, Evelyn Nguyen, Anton W. Neff

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The anuran Xenopus laevis is an experimental model for vertebrate development, immunology, and regenerative biology. Using histochemistry and immunohistochemistry (IHC) we examined embryonic, larval, and postmetamorphic Xenopus skin for the presence of dendritic cells (DCs), Langerhans cells (LCs), and dendritic epidermal T cells (DETCs), all components of cutaneous immunity that have been implicated in skin repair and regeneration. Cells expressing three markers for dendritic and Langerhans cells (formalin-resistant ATPase activity, major histocompatibility complex [MHC] class II antigens, and vimentin) and having morphology like that of these cells first appeared during late embryonic stages, becoming abundant by prometamorphosis. Cells positive for these markers were also numerous in the wound epithelia of regenerating hindlimbs at both early and late larval stages. Cells tentatively identified as DETCs were found, beginning at early larval stages, using IHC with antibodies against heterologous CD3ε chain and T-cell receptor δ. Further characterization and work with the putative DCs, LCs, and DETCs demonstrated here will allow not only greater understanding of the amphibian immune system, but also further elucidation of regenerative growth and scarring.

Original languageEnglish
Pages (from-to)383-393
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume31
Issue number4
DOIs
StatePublished - 2007

Fingerprint

Langerhans Cells
Xenopus
Regeneration
Immunity
Extremities
Skin
T-Lymphocytes
Dendritic Cells
Immunohistochemistry
Heterophile Antibodies
Histocompatibility Antigens Class II
Xenopus laevis
Amphibians
Vimentin
Cellular Structures
Hindlimb
T-Cell Antigen Receptor
Allergy and Immunology
Major Histocompatibility Complex
Formaldehyde

Keywords

  • Cutaneous immunity
  • Dendritic cells
  • DETCs
  • Langerhans cells
  • Limb regeneration
  • Scarring
  • Wound healing
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Cells of cutaneous immunity in Xenopus : Studies during larval development and limb regeneration. / Mescher, Anthony; Wolf, Wendy L.; Ashley Moseman, E.; Hartman, Byron; Harrison, Clint; Nguyen, Evelyn; Neff, Anton W.

In: Developmental and Comparative Immunology, Vol. 31, No. 4, 2007, p. 383-393.

Research output: Contribution to journalArticle

Mescher, Anthony ; Wolf, Wendy L. ; Ashley Moseman, E. ; Hartman, Byron ; Harrison, Clint ; Nguyen, Evelyn ; Neff, Anton W. / Cells of cutaneous immunity in Xenopus : Studies during larval development and limb regeneration. In: Developmental and Comparative Immunology. 2007 ; Vol. 31, No. 4. pp. 383-393.
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