Cellular control of in vitro progression of murine myeloid leukemia: Progression accompanies acquisition of independence from growth factor and stromal cells

H. Boswell, A. Srivastava, J. S. Burgess, P. Nahreini, N. Heerema, L. Inhorn, F. Padgett, E. B. Walker, R. W. Geib

Research output: Contribution to journalArticle

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Abstract

The ability of bone marrow stroma cells of normal WCB6F1(+/+) mice versus their congenic Sl/Sl(d) stromal-defective littermates to support sustained proliferation and leukemic transformation of the growth factor-dependent myeloid cell line FDC-P1 was studied. Extensive proliferation of factor-dependent cells occurred on (+/+) normal long-term marrow culture stroma without the addition of growth factor, whereas factor-dependent cells dissipated from Sl/Sl(d) stromal cultures after addition. The sustained proliferation that occurred on +/+ stromal layers later resulted in the appearance of factor-independent cell lines that were no longer dependent upon stroma. Factor-independent cell lines were cloned by limiting dilution and analyzed for expression of cell surface antigens to prove their origin from FDC-P1. Factor-independent cells, but not factor-dependent cells, formed tumors in syngeneic mice. These studies demonstrate a critical role for marrow stroma in the stepwise development of murine leukemia and are concordant with the previous data obtained in in vivo studies by McCool et al. that the splenic stroma of irradiated Sl/Sl(d) mice do not support growth of Friend virus-induced preleukemic cell colonies. The present data demonstrate in a preleukemia model not induced by Friend virus complex that normal (+/+) stromal cells promote the in vitro proliferation of factor-dependent preleukemic cells and their subsequent transition to factor-independent leukemia cells, but Sl/Sl(d) defective stroma do not efficiently promote this transition.

Original languageEnglish
Pages (from-to)765-771
Number of pages7
JournalLeukemia
Volume1
Issue number11
StatePublished - 1987

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Myeloid Leukemia
Stromal Cells
Intercellular Signaling Peptides and Proteins
Friend murine leukemia virus
Cell Line
Leukemia
Preleukemia
Bone Marrow
Myeloid Cells
Surface Antigens
In Vitro Techniques
Bone Marrow Cells
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Cellular control of in vitro progression of murine myeloid leukemia : Progression accompanies acquisition of independence from growth factor and stromal cells. / Boswell, H.; Srivastava, A.; Burgess, J. S.; Nahreini, P.; Heerema, N.; Inhorn, L.; Padgett, F.; Walker, E. B.; Geib, R. W.

In: Leukemia, Vol. 1, No. 11, 1987, p. 765-771.

Research output: Contribution to journalArticle

Boswell, H, Srivastava, A, Burgess, JS, Nahreini, P, Heerema, N, Inhorn, L, Padgett, F, Walker, EB & Geib, RW 1987, 'Cellular control of in vitro progression of murine myeloid leukemia: Progression accompanies acquisition of independence from growth factor and stromal cells', Leukemia, vol. 1, no. 11, pp. 765-771.
Boswell, H. ; Srivastava, A. ; Burgess, J. S. ; Nahreini, P. ; Heerema, N. ; Inhorn, L. ; Padgett, F. ; Walker, E. B. ; Geib, R. W. / Cellular control of in vitro progression of murine myeloid leukemia : Progression accompanies acquisition of independence from growth factor and stromal cells. In: Leukemia. 1987 ; Vol. 1, No. 11. pp. 765-771.
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