Cellular localization of androgen and estrogen receptors in mouse-derived motoneuron hybrid cells and mouse facial motoneurons

Julie Tetzlaff, Lisa Tanzer, Kathryn J. Jones

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The ability of gonadal steroid hormones to augment axonal regeneration after peripheral nerve injury has been well established in rat and hamster motoneuron systems, and provides a foundation for the use of these agents as neurotherapeutics. With the advent of mouse genetics and the availability of transgenic and knockout mice, the use of mice in studies of neuroprotection is growing. It has recently been demonstrated that both androgens and estrogens rescue motoneurons (MN) from injury in mouse-derived motoneuron hybrid cells in vitro and mouse facial motoneurons (FMN) in vivo (Tetzlaff et al. [2006] J Mol Neurosci 28:53-64). To elucidate the molecular mechanisms of these effects, the present study examined the cellular localization of androgen and estrogen receptors in mouse MN in vitro and in vivo. Immunoblotting and immunocytochemistry studies established the presence of androgen receptor (AR) and estrogen receptor α/β in immortalized mouse motoneuron hybrid cells and AR and estrogen receptor α in mouse FMN.

Original languageEnglish (US)
Pages (from-to)1362-1370
Number of pages9
JournalDevelopmental Neurobiology
Volume67
Issue number10
DOIs
StatePublished - Sep 1 2007

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Keywords

  • 17-β estradiol
  • Androgen receptor
  • Dihydrotestosterone
  • Estrogen receptor
  • Peripheral motor neuron

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

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