Cellular localization of tumor necrosis factor-α following acute spinal cord injury in adult rats

Ping Yan, Qun Li, Gyeong Moon Kim, Jian Xu, Chung Y. Hsu, Xiao-Ming Xu

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Posttraumatic inflammatory reaction may contribute to secondary injury after traumatic spinal cord injury (SCI). Expression of tumor necrosis factor-α (TNF-α), a key inflammatory mediator, has been demonstrated in the injured cord. However, the specific cell types that are responsible for TNF-α expression after SCI remain to be identified. In the present study, cellular sources of TNF-α were examined in rats that received a spinal cord impact injury at the 9th thoracic (T9) level. Here we demonstrate that, within hours after SCI, increased TNF-α immunoreactivity was localized in neurons, glial cells (including astrocytes, oligodendrocytes, and microglia), and endothelial cells in areas of the spinal cord adjacent to the lesion site. Myelin breakdown was noted in oligodendrocytes that are immunopositive for TNF-α. In sham-operated controls, a low level of TNF-α immunoreactivity was detected. In antigen-absorption experiments, no TNF-α immunoreactivity was detected, indicating the specificity of TNF-α immunocytochemistry in the present study. Results suggest that various cell types, including neurons, glial cells, and vascular endothelial cells, contribute to TNF-α production in the injured cord.

Original languageEnglish (US)
Pages (from-to)563-568
Number of pages6
JournalJournal of Neurotrauma
Volume18
Issue number5
StatePublished - May 2001
Externally publishedYes

Fingerprint

Spinal Cord Injuries
Tumor Necrosis Factor-alpha
Oligodendroglia
Neuroglia
Endothelial Cells
Neurons
Microglia
Myelin Sheath
Astrocytes
Spinal Cord
Thorax
Immunohistochemistry
Antigens
Wounds and Injuries

Keywords

  • Inflammation
  • Rat
  • Spinal cord injury
  • TNF-α

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Cellular localization of tumor necrosis factor-α following acute spinal cord injury in adult rats. / Yan, Ping; Li, Qun; Kim, Gyeong Moon; Xu, Jian; Hsu, Chung Y.; Xu, Xiao-Ming.

In: Journal of Neurotrauma, Vol. 18, No. 5, 05.2001, p. 563-568.

Research output: Contribution to journalArticle

Yan, Ping ; Li, Qun ; Kim, Gyeong Moon ; Xu, Jian ; Hsu, Chung Y. ; Xu, Xiao-Ming. / Cellular localization of tumor necrosis factor-α following acute spinal cord injury in adult rats. In: Journal of Neurotrauma. 2001 ; Vol. 18, No. 5. pp. 563-568.
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