Cellular mechanisms involved in mechanotransduction

R. Duncan, K. Brubaker, Xuening (Neal) Chen, N. Ajubi, K. Ryder, Fredrick Pavalko

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fluid flow induces increased c-fos and COX-2 expression in MC3T3-E1 cells that is dependent on flow-induced actin stress fiber formation (ASFF). The roles of intracellular Ca2+ ([Ca2+]i) and Ca2+ channels in these responses were examined using agents that alter [Ca2+]i release and Ca2+ entry. The intracellular Ca2+ chelator, BAPTA, abolished flow-induced ASFF and gene expression, but Ca2+ channel blockers, nifedipine and gadolinium, failed to inhibit these responses. Thapsigargin, which empties [Ca2+]i stores, and U73122, a phospholipase C inhibitor, completely suppressed flow-induced ASFF and c-fos/COX-2 expression. These data indicate that IP3-mediated [Ca2+]i release is essential for these flow induced responses in osteoblasts. However, we found that localization of Ca2+ channels to the membrane was required for the [Ca2+]i response to flow in MC3T3-E1 cells. Inhibition of these channels also reduced proliferation and increased alkaline phosphatase activity suggesting that these channels may be important to the differentiated state of osteoblastic cells.

Original languageEnglish
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Pages1303
Number of pages1
Volume2
ISBN (Print)0780356756
StatePublished - 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Actins
Fibers
Thapsigargin
Gadolinium
Osteoblasts
Phosphatases
Type C Phospholipases
Nifedipine
Chelating Agents
Gene expression
Alkaline Phosphatase
Flow of fluids
Membranes
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione

ASJC Scopus subject areas

  • Bioengineering

Cite this

Duncan, R., Brubaker, K., Chen, X. N., Ajubi, N., Ryder, K., & Pavalko, F. (1999). Cellular mechanisms involved in mechanotransduction. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 2, pp. 1303). IEEE.

Cellular mechanisms involved in mechanotransduction. / Duncan, R.; Brubaker, K.; Chen, Xuening (Neal); Ajubi, N.; Ryder, K.; Pavalko, Fredrick.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 2 IEEE, 1999. p. 1303.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Duncan, R, Brubaker, K, Chen, XN, Ajubi, N, Ryder, K & Pavalko, F 1999, Cellular mechanisms involved in mechanotransduction. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 2, IEEE, pp. 1303, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Duncan R, Brubaker K, Chen XN, Ajubi N, Ryder K, Pavalko F. Cellular mechanisms involved in mechanotransduction. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 2. IEEE. 1999. p. 1303
Duncan, R. ; Brubaker, K. ; Chen, Xuening (Neal) ; Ajubi, N. ; Ryder, K. ; Pavalko, Fredrick. / Cellular mechanisms involved in mechanotransduction. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 2 IEEE, 1999. pp. 1303
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