Cellulose acetate electrophoresis of hemoglobin

Ramesh Kumar, Wilbert Derbigny

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The electrophoresis on cellulose acetate membrane is most widely used because of its simplicity, and is without the use of any sophisticated instrument other than electrophoresis apparatus and the cellulose acetate strip. Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample. Sharp, clear bands without tailing effects can be obtained with this method. The method and apparatus described here would be appropriate to separate protein fractions under 1 h at voltages up to 60 V/cm measured between the electrodes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages81-85
Number of pages5
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1855
ISSN (Print)1064-3745

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Keywords

  • Amino acid
  • Electrophoresis
  • Membrane
  • Protein
  • Samples

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kumar, R., & Derbigny, W. (2019). Cellulose acetate electrophoresis of hemoglobin. In Methods in Molecular Biology (pp. 81-85). (Methods in Molecular Biology; Vol. 1855). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8793-1_7