CFU-GM-derived cells form osteoclasts at a very high efficiency

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The granulocyte-macrophage progenitor (CFU-GM) is a multipotent cell that can differentiate to osteoclasts (OCLs), macrophages, or granulocytes. However, the relative potential of CFU-GM to efficiently form OCLs is unknown. In this report we demonstrate that granulocyte-macrophage colony-forming unit (CFU-GM)-derived cells represent an easily obtainable highly purified source of human OCL precursors that form OCLs at very high efficiency (greater than 90%) when cultured with RANK ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and dexamethasone. The OCLs that formed have high bone-resorbing activity and form multiple resorption lacunae per OCL on dentin slices. Similarly, murine marrow-derived CFU-GM also formed OCLs at a high efficiency (> 80%) when treated with RANKL, M-CSF, and dexamethasone. In contrast, more committed macrophage colony-forming unit (CFU-M)-derived cells form few OCLs under these conditions. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)943-946
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume267
Issue number3
DOIs
StatePublished - Jan 27 2000
Externally publishedYes

Fingerprint

Granulocyte-Macrophage Progenitor Cells
Osteoclasts
Macrophages
RANK Ligand
Macrophage Colony-Stimulating Factor
Dexamethasone
Dentin
Granulocytes
Stem Cells
Bone Marrow

Keywords

  • Bone resorption
  • CFU-GM
  • Osteoclasts
  • Precursors

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

CFU-GM-derived cells form osteoclasts at a very high efficiency. / Menaa, Cheikh; Kurihara, Noriyoshi; Roodman, G. David.

In: Biochemical and Biophysical Research Communications, Vol. 267, No. 3, 27.01.2000, p. 943-946.

Research output: Contribution to journalArticle

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N2 - The granulocyte-macrophage progenitor (CFU-GM) is a multipotent cell that can differentiate to osteoclasts (OCLs), macrophages, or granulocytes. However, the relative potential of CFU-GM to efficiently form OCLs is unknown. In this report we demonstrate that granulocyte-macrophage colony-forming unit (CFU-GM)-derived cells represent an easily obtainable highly purified source of human OCL precursors that form OCLs at very high efficiency (greater than 90%) when cultured with RANK ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and dexamethasone. The OCLs that formed have high bone-resorbing activity and form multiple resorption lacunae per OCL on dentin slices. Similarly, murine marrow-derived CFU-GM also formed OCLs at a high efficiency (> 80%) when treated with RANKL, M-CSF, and dexamethasone. In contrast, more committed macrophage colony-forming unit (CFU-M)-derived cells form few OCLs under these conditions. (C) 2000 Academic Press.

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