Change in the degree of adsorption of proteins by aluminum-containing adjuvants following exposure to interstitial fluid: Freshly prepared and aged model vaccines

Yi Shi, Harm HogenEsch, Stanley L. Hem

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

The ability of interstitial fluid to change the degree of adsorption of ovalbumin to aluminum hydroxide adjuvant or lysozyme to aluminum phosphate adjuvant was studied. Ovalbumin and lysozyme were almost completely eluted after exposure at 37°C to sheep lymph fluid for 4h or 15min, respectively. The ability of sheep lymph fluid to elute lysozyme from aluminum phosphate adjuvant did not change as the model vaccine aged. However, only 60% of the ovalbumin adsorbed to aluminum hydroxide adjuvant was eluted during exposure to sheep lymph fluid for 24h after the model vaccine aged for 11 weeks at 4°C.

Original languageEnglish (US)
Pages (from-to)80-85
Number of pages6
JournalVaccine
Volume20
Issue number1-2
DOIs
StatePublished - Oct 12 2001
Externally publishedYes

Keywords

  • Aluminum adjuvants
  • Elution in interstitial fluid
  • Lysozyme
  • Ovalbumin

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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