Change in the degree of adsorption of proteins by aluminum-containing adjuvants following exposure to interstitial fluid

Freshly prepared and aged model vaccines

Yi Shi, Harm HogenEsch, Stanley L. Hem

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The ability of interstitial fluid to change the degree of adsorption of ovalbumin to aluminum hydroxide adjuvant or lysozyme to aluminum phosphate adjuvant was studied. Ovalbumin and lysozyme were almost completely eluted after exposure at 37°C to sheep lymph fluid for 4h or 15min, respectively. The ability of sheep lymph fluid to elute lysozyme from aluminum phosphate adjuvant did not change as the model vaccine aged. However, only 60% of the ovalbumin adsorbed to aluminum hydroxide adjuvant was eluted during exposure to sheep lymph fluid for 24h after the model vaccine aged for 11 weeks at 4°C.

Original languageEnglish (US)
Pages (from-to)80-85
Number of pages6
JournalVaccine
Volume20
Issue number1-2
DOIs
StatePublished - Oct 12 2001
Externally publishedYes

Fingerprint

extracellular fluids
Extracellular Fluid
Ovalbumin
Lymph
Muramidase
Aluminum
Adsorption
adjuvants
aluminum
lymph
Aluminum Hydroxide
Sheep
ovalbumin
adsorption
Vaccines
lysozyme
aluminum phosphate
vaccines
aluminum hydroxide
sheep

Keywords

  • Aluminum adjuvants
  • Elution in interstitial fluid
  • Lysozyme
  • Ovalbumin

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

@article{fda64887f41f4598bc3600c57cc8b6d9,
title = "Change in the degree of adsorption of proteins by aluminum-containing adjuvants following exposure to interstitial fluid: Freshly prepared and aged model vaccines",
abstract = "The ability of interstitial fluid to change the degree of adsorption of ovalbumin to aluminum hydroxide adjuvant or lysozyme to aluminum phosphate adjuvant was studied. Ovalbumin and lysozyme were almost completely eluted after exposure at 37°C to sheep lymph fluid for 4h or 15min, respectively. The ability of sheep lymph fluid to elute lysozyme from aluminum phosphate adjuvant did not change as the model vaccine aged. However, only 60{\%} of the ovalbumin adsorbed to aluminum hydroxide adjuvant was eluted during exposure to sheep lymph fluid for 24h after the model vaccine aged for 11 weeks at 4°C.",
keywords = "Aluminum adjuvants, Elution in interstitial fluid, Lysozyme, Ovalbumin",
author = "Yi Shi and Harm HogenEsch and Hem, {Stanley L.}",
year = "2001",
month = "10",
day = "12",
doi = "10.1016/S0264-410X(01)00313-9",
language = "English (US)",
volume = "20",
pages = "80--85",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "1-2",

}

TY - JOUR

T1 - Change in the degree of adsorption of proteins by aluminum-containing adjuvants following exposure to interstitial fluid

T2 - Freshly prepared and aged model vaccines

AU - Shi, Yi

AU - HogenEsch, Harm

AU - Hem, Stanley L.

PY - 2001/10/12

Y1 - 2001/10/12

N2 - The ability of interstitial fluid to change the degree of adsorption of ovalbumin to aluminum hydroxide adjuvant or lysozyme to aluminum phosphate adjuvant was studied. Ovalbumin and lysozyme were almost completely eluted after exposure at 37°C to sheep lymph fluid for 4h or 15min, respectively. The ability of sheep lymph fluid to elute lysozyme from aluminum phosphate adjuvant did not change as the model vaccine aged. However, only 60% of the ovalbumin adsorbed to aluminum hydroxide adjuvant was eluted during exposure to sheep lymph fluid for 24h after the model vaccine aged for 11 weeks at 4°C.

AB - The ability of interstitial fluid to change the degree of adsorption of ovalbumin to aluminum hydroxide adjuvant or lysozyme to aluminum phosphate adjuvant was studied. Ovalbumin and lysozyme were almost completely eluted after exposure at 37°C to sheep lymph fluid for 4h or 15min, respectively. The ability of sheep lymph fluid to elute lysozyme from aluminum phosphate adjuvant did not change as the model vaccine aged. However, only 60% of the ovalbumin adsorbed to aluminum hydroxide adjuvant was eluted during exposure to sheep lymph fluid for 24h after the model vaccine aged for 11 weeks at 4°C.

KW - Aluminum adjuvants

KW - Elution in interstitial fluid

KW - Lysozyme

KW - Ovalbumin

UR - http://www.scopus.com/inward/record.url?scp=0035850957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035850957&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(01)00313-9

DO - 10.1016/S0264-410X(01)00313-9

M3 - Article

VL - 20

SP - 80

EP - 85

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 1-2

ER -