Changes in B Cell Populations and Merozoite Surface Protein-1-Specific Memory B Cell Responses after Prolonged Absence of Detectable P. falciparum Infection

Cyrus Ayieko, Alexander C. Maue, Walter G Z O Jura, Gregory S. Noland, George Ayodo, Rosemary Rochford, Chandy John

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Clinical immunity to malaria declines in the absence of repeated parasite exposure. However, little is known about how B cell populations and antigen-specific memory B cells change in the absence of P. falciparum infection. A successful indoor residual insecticide spraying campaign in a highland area of western Kenya, led to an absence of blood-stage P. falciparum infection between March 2007 and April 2008. We assessed memory B cell responses in 45 adults at the beginning (April 2008) and end (April 2009) of a subsequent 12-month period during which none of the adults had evidence of asymptomatic parasitemia or clinical disease. Antibodies and memory B cells to the 42-kDa portion of the merozoite surface protein-1 (MSP-142) were measured using ELISA and ELISPOT assays, respectively. B cell populations were characterized by flow cytometry. From 2008 to 2009, the prevalence of MSP-142-specific memory B cells (45% vs. 55%, respectively, P = 0.32) or antibodies (91% vs. 82%, respectively, P = 0.32) did not differ significantly, although specific individuals did change from positive to negative and vice versa, particularly for memory B cells, suggesting possible low-level undetected parasitemia may have occurred in some individuals. The magnitude of MSP-142-specific memory B cells and levels of antibodies to MSP-142 also did not differ from 2008 to 2009 (P>0.10 for both). However, from 2008 to 2009 the proportions of both class-switched atypical (CD19+IgD-CD27-CD21-IgM-) and class-switched activated (CD19+IgD-CD27+CD21-IgM-) memory B cells decreased (both P42 specific memory B cells, but was associated with major changes in overall memory B cell subsets.

Original languageEnglish (US)
Article numbere67230
JournalPLoS One
Volume8
Issue number6
DOIs
StatePublished - Jun 27 2013
Externally publishedYes

Fingerprint

Merozoite Surface Protein 1
merozoites
surface proteins
B-lymphocytes
B-Lymphocytes
Cells
Data storage equipment
Infection
infection
Population
Immunoglobulin D
Parasitemia
parasitemia
Immunoglobulin M
Antibodies
antibodies
B-Lymphocyte Subsets
Enzyme-Linked Immunospot Assay
Kenya
Flow cytometry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Changes in B Cell Populations and Merozoite Surface Protein-1-Specific Memory B Cell Responses after Prolonged Absence of Detectable P. falciparum Infection. / Ayieko, Cyrus; Maue, Alexander C.; Jura, Walter G Z O; Noland, Gregory S.; Ayodo, George; Rochford, Rosemary; John, Chandy.

In: PLoS One, Vol. 8, No. 6, e67230, 27.06.2013.

Research output: Contribution to journalArticle

Ayieko, Cyrus ; Maue, Alexander C. ; Jura, Walter G Z O ; Noland, Gregory S. ; Ayodo, George ; Rochford, Rosemary ; John, Chandy. / Changes in B Cell Populations and Merozoite Surface Protein-1-Specific Memory B Cell Responses after Prolonged Absence of Detectable P. falciparum Infection. In: PLoS One. 2013 ; Vol. 8, No. 6.
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