Changes in gene and protein expression in magnetic field-treated human glioma cells

R. E. Savage, M. H. Kanitz, W. G. Lotz, D. Conover, E. M. Hehnessey, W. H. Hanneman, F. A. Witzmann

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Because few cancer studies have examined protein profiles and genetic regulation from a single carcinogen exposure, the objective of this study was to determine genetic change via microarray and to evaluate whether that change was a precursor to cellular protein changes. In separate but experimentally identical studies, human glioma SF767 cells were exposed for 3 h to 60-Hz magnetic fields (sham or 1.2 μT). Microarray results suggested that magnetic field treatment resulted in the up-regulation of 5 genes, whereas 25 genes were down-regulated. The mean abundance of 10 identified proteins was altered following 1.2 μT exposure relative to sham (3 increase, 7 decrease). These studies suggest a limited but complicated response in the glioma cells to the magnetic field treatment.

Original languageEnglish (US)
Pages (from-to)115-120
Number of pages6
JournalToxicology Mechanisms and Methods
Volume15
Issue number2
DOIs
StatePublished - Mar 1 2005

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Keywords

  • Genomics
  • Glioma
  • Magnetic Field
  • Proteomics

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Savage, R. E., Kanitz, M. H., Lotz, W. G., Conover, D., Hehnessey, E. M., Hanneman, W. H., & Witzmann, F. A. (2005). Changes in gene and protein expression in magnetic field-treated human glioma cells. Toxicology Mechanisms and Methods, 15(2), 115-120. https://doi.org/10.1080/15376520590918829