Changes in gene and protein expression in magnetic field-treated human glioma cells

R. E. Savage, M. H. Kanitz, W. G. Lotz, D. Conover, E. M. Hehnessey, W. H. Hanneman, Frank Witzmann

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Because few cancer studies have examined protein profiles and genetic regulation from a single carcinogen exposure, the objective of this study was to determine genetic change via microarray and to evaluate whether that change was a precursor to cellular protein changes. In separate but experimentally identical studies, human glioma SF767 cells were exposed for 3 h to 60-Hz magnetic fields (sham or 1.2 μT). Microarray results suggested that magnetic field treatment resulted in the up-regulation of 5 genes, whereas 25 genes were down-regulated. The mean abundance of 10 identified proteins was altered following 1.2 μT exposure relative to sham (3 increase, 7 decrease). These studies suggest a limited but complicated response in the glioma cells to the magnetic field treatment.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalToxicology Mechanisms and Methods
Volume15
Issue number2
DOIs
StatePublished - Mar 2005

Fingerprint

Magnetic Fields
Glioma
Microarrays
Magnetic fields
Gene Expression
Genes
Proteins
Carcinogens
Up-Regulation
Neoplasms

Keywords

  • Genomics
  • Glioma
  • Magnetic Field
  • Proteomics

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Savage, R. E., Kanitz, M. H., Lotz, W. G., Conover, D., Hehnessey, E. M., Hanneman, W. H., & Witzmann, F. (2005). Changes in gene and protein expression in magnetic field-treated human glioma cells. Toxicology Mechanisms and Methods, 15(2), 115-120. https://doi.org/10.1080/15376520590918829

Changes in gene and protein expression in magnetic field-treated human glioma cells. / Savage, R. E.; Kanitz, M. H.; Lotz, W. G.; Conover, D.; Hehnessey, E. M.; Hanneman, W. H.; Witzmann, Frank.

In: Toxicology Mechanisms and Methods, Vol. 15, No. 2, 03.2005, p. 115-120.

Research output: Contribution to journalArticle

Savage, RE, Kanitz, MH, Lotz, WG, Conover, D, Hehnessey, EM, Hanneman, WH & Witzmann, F 2005, 'Changes in gene and protein expression in magnetic field-treated human glioma cells', Toxicology Mechanisms and Methods, vol. 15, no. 2, pp. 115-120. https://doi.org/10.1080/15376520590918829
Savage, R. E. ; Kanitz, M. H. ; Lotz, W. G. ; Conover, D. ; Hehnessey, E. M. ; Hanneman, W. H. ; Witzmann, Frank. / Changes in gene and protein expression in magnetic field-treated human glioma cells. In: Toxicology Mechanisms and Methods. 2005 ; Vol. 15, No. 2. pp. 115-120.
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