Changes in gene expression within the extended amygdala following binge-like alcohol drinking by adolescent alcohol-preferring (P) rats

William J. McBride, Mark W. Kimpel, Jeanette McClintick, Zheng Ming Ding, Howard Edenberg, Tiebing Liang, Zachary Rodd, Richard Bell

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The objective of this study was to determine changes in gene expression within the extended amygdala following binge-like alcohol drinking by male adolescent alcohol-preferring (P) rats. Starting at 28 days of age, P rats were given concurrent access to 15 and 30% ethanol for 3 one-h sessions/day for 5 consecutive days/week for 3 weeks. Rats were killed by decapitation 3 h after the first ethanol access session on the 15th day of drinking. RNA was prepared from micropunch samples of the nucleus accumbens shell (Acb-sh) and central nucleus of the amygdala (CeA). Ethanol intakes were 2.5-3.0 g/kg/session. There were 154 and 182 unique named genes that significantly differed (FDR = 0.2) between the water and ethanol group in the Acb-sh and CeA, respectively. Gene Ontology (GO) analyses indicated that adolescent binge drinking produced changes in biological processes involved with cell proliferation and regulation of cellular structure in the Acb-sh, and in neuron projection and positive regulation of cellular organization in the CeA. Ingenuity Pathway Analysis indicated that, in the Acb-sh, there were several major intracellular signaling pathways (e.g., cAMP-mediated and protein kinase A signaling pathways) altered by adolescent drinking, with 3-fold more genes up-regulated than down-regulated in the alcohol group. The cAMP-mediated signaling system was also up-regulated in the CeA of the alcohol group. Weighted gene co-expression network analysis indicated significant G-protein coupled receptor signaling and transmembrane receptor protein kinase signaling categories in the Acb-sh and CeA, respectively. Overall, the results of this study indicated that binge-like alcohol drinking by adolescent P rats is differentially altering the expression of genes in the Acb-sh and CeA, some of which are involved in intracellular signaling pathways and may produce changes in neuronal function.

Original languageEnglish (US)
Pages (from-to)52-60
Number of pages9
JournalPharmacology Biochemistry and Behavior
Volume117
DOIs
StatePublished - 2014

Fingerprint

Amygdala
Gene expression
Alcohol Drinking
Rats
Alcohols
Gene Expression
Genes
Ethanol
Binge Drinking
Decapitation
Biological Phenomena
Gene Ontology
Nucleus Accumbens
Cell proliferation
Cellular Structures
Electric network analysis
G-Protein-Coupled Receptors
Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Drinking

Keywords

  • Adolescent binge drinking
  • Alcohol-preferring rat
  • Central nucleus of the amygdala
  • Gene expression
  • Nucleus accumbens-shell

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Pharmacology
  • Toxicology
  • Behavioral Neuroscience
  • Biological Psychiatry

Cite this

Changes in gene expression within the extended amygdala following binge-like alcohol drinking by adolescent alcohol-preferring (P) rats. / McBride, William J.; Kimpel, Mark W.; McClintick, Jeanette; Ding, Zheng Ming; Edenberg, Howard; Liang, Tiebing; Rodd, Zachary; Bell, Richard.

In: Pharmacology Biochemistry and Behavior, Vol. 117, 2014, p. 52-60.

Research output: Contribution to journalArticle

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