Changes in insulin-like growth factor-binding proteins in bovine mammary secretions associated with pregnancy and parturition

Todd Skaar, J. R. Vega, S. N. Pyke, C. R. Baumrucker

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Abstract

The bovine mammary gland accumulates large quantities of insulin-like growth factors (IGFs) and IGF-binding proteins (IGFBPs) during late gestation which are secreted at parturition. The present study was conducted to determine the changes in the profiles of IGFBPs secreted by the mammary gland and in blood during late gestation and early lactation in dairy cows. Ligand blotting of serum and mammary secretions showed that IGFBPs of M(r) 25,000, 30,000, 34,000, 42,000, 46,000 and >200,000 were present in both fluids. The binding activity of the 42-46,000 M(r) IGFBP predominated in prepartum mammary secretions and colostrum but was reduced postpartum. The binding activities of the 30,000 and 34,000 M(r) IGFBPs, relative to other IGFBPs, were increased postpartum. Concentrations of IGF-I and IGF-II in mammary secretions declined from 347.1 and 181.1 nmol/litre 1 week prepartum to 0.7 and 0.3 nmol/litre 1.5 weeks postpartum. The volume of mammary secretions obtained was 0.109 litre and 6.690 litres at 1 week prepartum and 1.5 weeks postpartum respectively. In prepartum serum, the greatest binding activity was at M(r) 42-46,000. The activity at this M(r) decreased at parturition but was restored postpartum. The binding activities of the 30,000 and 34,000 M(r) IGFBPs were increased around parturition. The 25,000 M(r) IGFBP had minor activity during all periods. IGF-I concentrations decreased from 10.6 nmol/litres 1 week prepartum to 4.7 nmol/litres 1.5 weeks postpartum but IGF-II concentrations remained constant. In conclusion, IGFBP activity secreted by the mammary gland shifts from primarily M(r) 42-46,000 prepartum to M(r) 30,000 postpartum. The 42-46,000 M(r) IGFBP activity in serum was not abolished during late gestation as reported in several monogastric species.

Original languageEnglish (US)
Pages (from-to)127-133
Number of pages7
JournalJournal of Endocrinology
Volume131
Issue number1
StatePublished - 1991
Externally publishedYes

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Insulin-Like Growth Factor Binding Proteins
Breast
Parturition
Postpartum Period
Pregnancy
Human Mammary Glands
Insulin-Like Growth Factor II
Insulin-Like Growth Factor I
Serum
Colostrum
Somatomedins
Lactation
Ligands

ASJC Scopus subject areas

  • Endocrinology

Cite this

Changes in insulin-like growth factor-binding proteins in bovine mammary secretions associated with pregnancy and parturition. / Skaar, Todd; Vega, J. R.; Pyke, S. N.; Baumrucker, C. R.

In: Journal of Endocrinology, Vol. 131, No. 1, 1991, p. 127-133.

Research output: Contribution to journalArticle

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abstract = "The bovine mammary gland accumulates large quantities of insulin-like growth factors (IGFs) and IGF-binding proteins (IGFBPs) during late gestation which are secreted at parturition. The present study was conducted to determine the changes in the profiles of IGFBPs secreted by the mammary gland and in blood during late gestation and early lactation in dairy cows. Ligand blotting of serum and mammary secretions showed that IGFBPs of M(r) 25,000, 30,000, 34,000, 42,000, 46,000 and >200,000 were present in both fluids. The binding activity of the 42-46,000 M(r) IGFBP predominated in prepartum mammary secretions and colostrum but was reduced postpartum. The binding activities of the 30,000 and 34,000 M(r) IGFBPs, relative to other IGFBPs, were increased postpartum. Concentrations of IGF-I and IGF-II in mammary secretions declined from 347.1 and 181.1 nmol/litre 1 week prepartum to 0.7 and 0.3 nmol/litre 1.5 weeks postpartum. The volume of mammary secretions obtained was 0.109 litre and 6.690 litres at 1 week prepartum and 1.5 weeks postpartum respectively. In prepartum serum, the greatest binding activity was at M(r) 42-46,000. The activity at this M(r) decreased at parturition but was restored postpartum. The binding activities of the 30,000 and 34,000 M(r) IGFBPs were increased around parturition. The 25,000 M(r) IGFBP had minor activity during all periods. IGF-I concentrations decreased from 10.6 nmol/litres 1 week prepartum to 4.7 nmol/litres 1.5 weeks postpartum but IGF-II concentrations remained constant. In conclusion, IGFBP activity secreted by the mammary gland shifts from primarily M(r) 42-46,000 prepartum to M(r) 30,000 postpartum. The 42-46,000 M(r) IGFBP activity in serum was not abolished during late gestation as reported in several monogastric species.",
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