Changes in insulin-like growth factors I and II and their binding protein after a single intramuscular injection of growth hormone

M. B. Grant, I. Schmetz, B. Russell, H. J. Harwood, J. Silverstein, T. J. Merimee

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Abstract

The concentrations of insulin-like growth factors I and II (IGF-I and IGF-II) and their binding proteins in serum were measured in 10 GH-deficient patients before and after a single 6-IU injection of GH. Serum IGF-I concentrations were initially low, increased significantly by 8 and 24 h, and decreased to pretreatment levels 48 and 72 h after GH administration. Serum IGF-II concentrations also were low initially and did not increase by 8 and 24 h, but were however, significantly higher 48 and 72 h after GH administration. In GH-deficient patients before GH administration, binding of IGF-I or IGF-II to serum proteins were restricted primarily to proteins of 50K mol wt. Little or no binding to proteins of 150,000 mol wt was found. By 8 and 24 h after GH injection, IGF-I, but not IGF-II, bound primarily to a protein(s) of 150K mol wt, as in normal subjects. IGF-II remained bound to a 50K mol wt protein. By 48 and 72 h after administering GH, however, the binding pattern was reversed, and IGF-II, but not IGF-I, bound predominantly to a protein(s) of 150K mol wt. Our data demonstrate both a temporal dissociation in the responses of IGF-I and IGF-II to GH and a similar temporal dissociation in the binding of IGF-I and IGF-II to the large mol wt (150K) binding protein. This dissociation, particularly the latter, may provide a means for better characterization of protein fractions in binding IGF, particularly in terms of specificity.

Original languageEnglish (US)
Pages (from-to)981-984
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume63
Issue number4
StatePublished - 1986
Externally publishedYes

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Insulin-Like Growth Factor II
Intramuscular Injections
Insulin-Like Growth Factor I
Growth Hormone
Carrier Proteins
Proteins
Serum
Injections
Blood Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Changes in insulin-like growth factors I and II and their binding protein after a single intramuscular injection of growth hormone. / Grant, M. B.; Schmetz, I.; Russell, B.; Harwood, H. J.; Silverstein, J.; Merimee, T. J.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 63, No. 4, 1986, p. 981-984.

Research output: Contribution to journalArticle

Grant, M. B. ; Schmetz, I. ; Russell, B. ; Harwood, H. J. ; Silverstein, J. ; Merimee, T. J. / Changes in insulin-like growth factors I and II and their binding protein after a single intramuscular injection of growth hormone. In: Journal of Clinical Endocrinology and Metabolism. 1986 ; Vol. 63, No. 4. pp. 981-984.
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