Changes in intracellular free calcium during hyperthermia: Effects of local anesthetics and induction of thermotolerance

Joseph Dynlacht, W. C. Hyun, W. C. Dewey

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We wished to determine if local anesthetics (LAs) induced changes in intracellular free calcium ([fCa2+(i)]) that could have an effect on cell killing by hyperthermia. Flow cytometry was used to measure [fCa2+(i)] of mouse NIH 3T3 cells during heating at 45.5°C. In both non-tolerant and thermotolerant cells, heating caused a rapid increase (within 1 min) in [fCa2+(i)] of ~100 nM, which remained relatively constant during 25 min of continued heating; however, survival was higher in thermotolerant cells. Procaine, lidocaine, and tetracaine had no effect on survival or [fCa2+(i)] of cells kept at 37°C up to 25 min. Cells heated with procaine and lidocaine showed no difference in [fCa2+(i)] compared to cells heated without LAs but were greatly sensitized to killing. Cells heated with tetracine became permeable to trypan blue within 10-15 min of heating. We conclude that heat sensitization by LAs does not involve changes in [fCa2+(i)]. Furthermore, these studies reject the hypothesis that changes in [fCa2+(i)] are involved in heat-induced cell killing.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalCytometry
Volume14
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Local Anesthetics
Fever
Calcium
Heating
Procaine
Lidocaine
Hot Temperature
Tetracaine
NIH 3T3 Cells
Trypan Blue
Thermotolerance
Flow Cytometry

Keywords

  • Flow cytometry
  • Indol-1
  • Lidocaine
  • Procaine
  • Tetracaine

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Changes in intracellular free calcium during hyperthermia : Effects of local anesthetics and induction of thermotolerance. / Dynlacht, Joseph; Hyun, W. C.; Dewey, W. C.

In: Cytometry, Vol. 14, No. 2, 1993, p. 223-229.

Research output: Contribution to journalArticle

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