Changes in intracellular free calcium during hyperthermia: Effects of local anesthetics and induction of thermotolerance

Joseph R. Dynlacht, William C. Hyun, William C. Dewey

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We wished to determine if local anesthetics (LAs) induced changes in intracellular free calcium ([fCa2+i]) that could have an effect on cell killing by hyperthermia. Flow cytometry was used to measure [fCa2+i] of mouse NIH 3T3 cells during heating at 45.5°C. In both non‐tolerant and thermotolerant cells, heating caused a rapid increase (within 1 min) in [fCa2+i] of ∼100 nM, which remained relatively constant during 25 min of continued heating; however, survival was higher in thermotolerant cells. Procaine, lidocaine, and tetracaine had no effect on survival or [fCa2+i] of cells kept at 37°C up to 25 min. Cells heated with procaine and lidocaine showed no difference in [fCa2+i] compared to cells heated without LAs but were greatly sensitized to killing. Cells heated with tetracaine became permeable to trypan blue within 10–15 min of heating. We conclude that heat sensitization by LAs does not involve changes in [fCa2+i]. Furthermore, these studies reject the hypothesis that changes in [fCa2+i] are involved in heat‐induced cell killing. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalCytometry
Volume14
Issue number2
DOIs
StatePublished - 1993

Keywords

  • Flow cytometry
  • Indo‐1
  • lidocaine
  • procaine
  • tetracaine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

Fingerprint Dive into the research topics of 'Changes in intracellular free calcium during hyperthermia: Effects of local anesthetics and induction of thermotolerance'. Together they form a unique fingerprint.

Cite this