Changes in liver protein abundance in inbred alcohol-preferring rats due to chronic alcohol exposure, as measured through a proteomics approach

Iveta Klouckova, Petra Hrncirova, Yehia Mechref, Randy J. Arnold, Ting Kai Li, William J. McBride, Milos V. Novotny

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This study compares the total liver proteome of inbred alcohol-preferring line (iP) rats exposed to alcohol with iP rats without alcohol experience. Rat liver proteins were extracted using a three-step procedure. Each of the three solutions solubilizes a different set of proteins. The extracted proteins were separated by 2-DE. Scanned gels of two sample groups, alcohol-exposed iP and alcohol-naive iP, were compared, revealing many protein spots with significantly higher or lower densities. These spots were cut from the gel, destained, and subjected to trypsin digestion and subsequent identification by LC-MS/MS. Twenty-four individual rats, 12 alcohol-naive, and 12 alcohol-exposed, were used in this study. Two groups, each containing six naïve and six exposed animals, were created for statistical comparison. For the first group, 64 spots were observed to have statistically significant intensity differences upon alcohol exposure across all three extracts while 118 such spots were found in the second group. There were 113 unique proteins in both groups together. The majority of these proteins were enzymes. Significant changes are observed for three major metabolic pathways: glycolysis, gluconeogenesis, and fatty acid β-oxidation. In addition, enzymes involved in protein synthesis and antioxidant activity show significant changes in abundance in response to alcohol exposure.

Original languageEnglish
Pages (from-to)3060-3074
Number of pages15
JournalProteomics
Volume6
Issue number10
DOIs
StatePublished - May 2006

Fingerprint

Liver
Proteomics
Rats
Alcohols
Proteins
Gels
Gluconeogenesis
Glycolysis
Enzymes
Proteome
Metabolic Networks and Pathways
Trypsin
Digestion
Animals
Fatty Acids
Antioxidants
Oxidation

Keywords

  • Alcohol dependency
  • LC-MS/MS
  • Rat liver
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Klouckova, I., Hrncirova, P., Mechref, Y., Arnold, R. J., Li, T. K., McBride, W. J., & Novotny, M. V. (2006). Changes in liver protein abundance in inbred alcohol-preferring rats due to chronic alcohol exposure, as measured through a proteomics approach. Proteomics, 6(10), 3060-3074. https://doi.org/10.1002/pmic.200500725

Changes in liver protein abundance in inbred alcohol-preferring rats due to chronic alcohol exposure, as measured through a proteomics approach. / Klouckova, Iveta; Hrncirova, Petra; Mechref, Yehia; Arnold, Randy J.; Li, Ting Kai; McBride, William J.; Novotny, Milos V.

In: Proteomics, Vol. 6, No. 10, 05.2006, p. 3060-3074.

Research output: Contribution to journalArticle

Klouckova, I, Hrncirova, P, Mechref, Y, Arnold, RJ, Li, TK, McBride, WJ & Novotny, MV 2006, 'Changes in liver protein abundance in inbred alcohol-preferring rats due to chronic alcohol exposure, as measured through a proteomics approach', Proteomics, vol. 6, no. 10, pp. 3060-3074. https://doi.org/10.1002/pmic.200500725
Klouckova, Iveta ; Hrncirova, Petra ; Mechref, Yehia ; Arnold, Randy J. ; Li, Ting Kai ; McBride, William J. ; Novotny, Milos V. / Changes in liver protein abundance in inbred alcohol-preferring rats due to chronic alcohol exposure, as measured through a proteomics approach. In: Proteomics. 2006 ; Vol. 6, No. 10. pp. 3060-3074.
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