Changes in membrane properties of CA1 pyramidal neurons after transient forebrain ischemia in vivo

T. M. Gao, W. A. Pulsinelli, Z. C. Xu

Research output: Contribution to journalArticle

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Abstract

We have previously identified three distinct populations of CA1 pyramidal neurons after reperfusion based on differences in synaptic response, and named these late depolarizing postsynaptic potential neurons (enhanced synaptic transmission), non-late depolarizing postsynaptic potential and small excitatory postsynaptic neurons (depressed synaptic transmission). In the present study, spontaneous activity and membrane properties of CA1 neurons were examined up to 48 h following ~ 14 min ischemic depolarization using intracellular recording and staining techniques in vivo. In comparison with preischemic properties, the spontaneous firing rate and the spontaneous synaptic activity of CA1 neurons decreased significantly during reperfusion; spontaneous synaptic activity ceased completely 36-48 h after reperfusion, except for a low level of activity which persisted in non-late depolarizing postsynaptic potential neurons. Neuronal hyperactivity as indicated by increasing firing rate was never observed in the present study. The membrane input resistance and time constant decreased significantly in late depolarizing postsynaptic potential neurons at 24-48 h reperfusion. In contrast, similar changes were not observed in non-late depolarizing postsynaptic potential neurons. The rheobase, spike threshold and spike frequency adaptation in late depolarizing postsynaptic potential neurons increased progressively following reperfusion. Only a transient increase in rheobase and spike threshold was detected in non-late depolarizing postsynaptic potential neurons and spike frequency adaptation remained unchanged in these neurons. The amplitude of fast afterhyperpolarization increased in all neurons after reperfusion, with the smallest increment in non-late depolarizing postsynaptic potential neurons. Small excitatory postsynaptic potential neurons shared similar changes to those of late depolarizing postsynaptic potential neurons. These results suggest that the enhancement and depression of synaptic transmission following ischemia are probably due to changes in synaptic efficacy rather than changes in intrinsic membrane properties. The neurons with enhanced synaptic transmission following ischemia are probably the degenerating neurons, while the neurons with depressed synaptic transmission may survive the ischemic insult.

Original languageEnglish (US)
Pages (from-to)771-780
Number of pages10
JournalNeuroscience
Volume90
Issue number3
DOIs
StatePublished - May 1999
Externally publishedYes

Fingerprint

Pyramidal Cells
Prosencephalon
Ischemia
Neurons
Membranes
Synaptic Potentials
Reperfusion
Synaptic Transmission
Excitatory Postsynaptic Potentials

Keywords

  • Electrophysiology
  • Hippocampus
  • In vivo intracellular recording
  • Ischemia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Changes in membrane properties of CA1 pyramidal neurons after transient forebrain ischemia in vivo. / Gao, T. M.; Pulsinelli, W. A.; Xu, Z. C.

In: Neuroscience, Vol. 90, No. 3, 05.1999, p. 771-780.

Research output: Contribution to journalArticle

Gao, T. M. ; Pulsinelli, W. A. ; Xu, Z. C. / Changes in membrane properties of CA1 pyramidal neurons after transient forebrain ischemia in vivo. In: Neuroscience. 1999 ; Vol. 90, No. 3. pp. 771-780.
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