Changes in nuclear envelope invaginations in axotomized immature and mature hamster facial motoneurons

Kathryn J. Jones, Arthur LaVelle

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

In this study, changes in the amount of nuclear envelope invaginations (NEI) were quantitatively assessed after axotomy during the late nuclear maturation stages (15, 20 and 25 days postnatal age) and in the adult (100-day-old) hamster facial motoneurons. These changes were expressed as boundary density or BA (length of nuclear envelope per unit area of nucleus). Absolute nuclear areas and perimeters were also estimated after axotomy at these ages. At 1 2 and 1 day after axotomy, no differences in the above parameters were noted at any of the operative ages. At 4 days postoperative, the peak of chromatolysis for all these ages, axotomy resulted in significant decreases in BA and nuclear perimeter in the immature neurons and no changes in BA and nuclear perimeter in the adult neurons. In addition, 4 days after axotomy at 20 days postnatal and later ages, pronounced increases in nuclear area occurred. These quantitative results are interpreted as evidence that the accelerated loss of NEI after axotomy during the final stages of nuclear maturation in these neurons is related to the formation of rough endoplasmic reticulum (RER) or Nissl substance. The hypothesis that an 'excess' of RER is accumulated during the late maturation stages and may account for the lack of NEI in the adult axotomized facial motoneuron is presented.

Original languageEnglish (US)
Pages (from-to)241-249
Number of pages9
JournalDevelopmental Brain Research
Volume21
Issue number2
DOIs
StatePublished - Aug 1985

    Fingerprint

Keywords

  • axotomy
  • development
  • facial motoneuron
  • hamster
  • morphometry
  • nuclear envelope invagination

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

Cite this