Changes in salivary microbial sensing proteins CD14 and TLR2 with aging

Sable Staller, Alison K. Lindsay, Elizabeth D. Ramos, Priya Thomas, Mythily Srinivasan

Research output: Contribution to journalArticle


Objective: Soluble toll-like receptor-2 (sTLR2) and soluble CD14 (sCD14) in saliva are defense proteins that bind specific microbe-associated molecular patterns. Since the oral flora changes with aging, the objective of this study is to determine and compare the concentration of sTLR2 and sCD14 in the saliva of healthy individuals in age groups from the first to the sixth decade of life. Methods: Unstimulated whole saliva was collected after obtaining informed consent. The concentration of sCD14 and sTLR-2 was measured by enzyme-linked immunosorbent assay. Statistical differences between the age groups were determined by analysis of variance. The relationship between the two markers in each age group was evaluated by Pearson’s correlation coefficient and linear regression analyses. Results: The concentration of salivary sTLR2 was highest in the youngest, and that of the sCD14 was highest in the oldest age group. While the salivary sCD14 and the sTLR2 exhibited a moderate negative correlation in the youngest, the relationship between the two markers was inversed in the oldest age group. Conclusions and clinical relevance: The results of our exploratory study suggest a need to adjust for age-dependent changes in sCD14 and sTLR2 in healthy saliva while assessing the two proteins as biomarkers.

Original languageEnglish (US)
Pages (from-to)2523-2528
Number of pages6
JournalClinical oral investigations
Issue number7
StatePublished - Jul 1 2020
Externally publishedYes


  • Age changes
  • CD14
  • Saliva
  • Toll-like receptor-2

ASJC Scopus subject areas

  • Dentistry(all)

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