Changes in the extracellular matrix and glycosaminoglycan synthesis during the initiation of regeneration in adult newt forelimbs

Anthony Mescher, S. I. Munaim

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The extracellular matrix (ECM) of the distal tissues in a newt limb stump is completely reorganized in the 2-3 week period following amputation. In view of numerous in vitro studies showing that extracellular material influences cellular migration and proliferation, it is likely that the changes in the limb's ECM are important activities in the process leading to regeneration of such limbs. Using biochemical, autoradiographic, and histochemical techniques we studied temporal and spatial differences in the synthesis of glycosaminoglycans (GAGs) during the early, nerve-dependent phase of limb regeneration. Hyaluronic acid synthesis began with the onset of tissue dedifferentiation, became maximal within 1 week, and continued throughout the period of active cell proliferation. Chondroitin sulfate synthesis began somewhat later, increased steadily, and reached very high levels during chondrogenesis. During the first 10 days after amputation, distributions of sulfated and nonsulfated GAGs were both uniform throughout dedifferentiating tissues, except for a heavier localization near the bone. Since nerves are necessary to promote the regenerative process, we examined the neural influence on synthesis and accumulation of extracellular GAGs. Denervation decreased GAG production in all parts of the limb stump by approximately 50%. Newt dorsal root ganglia and brain-derived fibroblast growth factor each produced twofold stimulation of GAG synthesis of hyaluronic acid. The results indicate that the trophic action of nerves on amphibian limb regeneration includes a positive influence on synthesis and extracellular accumulation of GAGs. Since the ECM exerts a major influence on cellular proliferation and migration, the effect of nerves on GAG metabolism may have considerable importance for growth and development of the early regenerate.

Original languageEnglish
Pages (from-to)424-431
Number of pages8
JournalAnatomical Record
Volume214
Issue number4
StatePublished - 1986

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Salamandridae
Forelimb
glycosaminoglycans
forelimbs
Glycosaminoglycans
salamanders and newts
extracellular matrix
Extracellular Matrix
limbs (animal)
Regeneration
Extremities
synthesis
nerve tissue
A73025
hyaluronic acid
Cell Proliferation
cell proliferation
Hyaluronic Acid
amputation
Amputation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Changes in the extracellular matrix and glycosaminoglycan synthesis during the initiation of regeneration in adult newt forelimbs. / Mescher, Anthony; Munaim, S. I.

In: Anatomical Record, Vol. 214, No. 4, 1986, p. 424-431.

Research output: Contribution to journalArticle

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