Changing spatial patterns of DNA replication in the noise-damaged chick cochlea

Eri Hashino, Richard J. Salvi

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The purpose of the present study was to examine the spatio-temporal pattern of cell proliferation in the chick cochlea in response to the sensory hair cell loss induced by a 1.5 kHz pure tone at 120 dB SPL (1 dB=20 μPa) for 48 h. DNA replication was evaluated with the bromodeoxyuridine (BrdU) pulse-fix technique. One group of birds was given multiple injections of BrdU (50 mg/kg) over a period of 8 h at various starting times during or after the exposure. Afterwards, their cochleas were removed and processed as whole mounts for BrdU immunohistochemistry. The cochleas of a second group of acoustically traumatized chicks were evaluated by scanning electron microscopy in order to determine the spatio-temporal pattern of hair cell loss. Hair cell loss was first observed 12 h after the start of the exposure and DNA replication started near the inferior edge of the hair cell lesion 24-32 h after the start of the exposure, i.e. 12-20 h after the first sign of hair cell loss. The site of hair cell loss and DNA replication shifted toward the superior edge of the basilar papilla as the exposure continued. The rate of DNA replication accelerated and reached its peak near the end of the 48 h exposure. The estimated latency of cell proliferation after hair cell loss was faster and the duration of DNA replication shorter than that observed in other sensory systems. The spatio-temporal pattern of DNA replication follows the spatio-temporal gradient of hair cell loss, suggesting that cell proliferation is triggered by hair cell loss itself rather than by intrinsic positional cues or gradients.

Original languageEnglish (US)
Pages (from-to)23-31
Number of pages9
JournalJournal of Cell Science
Volume105
Issue number1
StatePublished - May 1993
Externally publishedYes

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Cochlea
DNA Replication
Alopecia
Noise
Bromodeoxyuridine
Cell Proliferation
DNA Replication Timing
Organ of Corti
Electron Scanning Microscopy
Birds
Cues
Immunohistochemistry
Injections

Keywords

  • DNA replication
  • Hair cell
  • Regeneration

ASJC Scopus subject areas

  • Cell Biology

Cite this

Changing spatial patterns of DNA replication in the noise-damaged chick cochlea. / Hashino, Eri; Salvi, Richard J.

In: Journal of Cell Science, Vol. 105, No. 1, 05.1993, p. 23-31.

Research output: Contribution to journalArticle

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