Characteristics of activated monocyte phenotype support R5-tropic human immunodeficiency virus

Sody M. Munsaka, Melissa Agsalda, David Troelstrup, Ningjie Hu, Andy Yu, Bruce Shiramizu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Microbial translocation has been recognized as an important factor in monocyte activation and contributing to AIDS pathogenesis with elevated plasma lipopolysaccharide (LPS) levels, as a marker for microbial translocation, seen in advanced HIV disease. Therefore, the current study was undertaken to assess monocyte activation in vitro by LPS and to determine its impact on monocyte phenotype. Methods: Monocytes from non-HIV-infected donors were analyzed for CD14, CD16, CD69, TNFα, and CCR5 by flow cytometry pre- and post-stimulation with LPS. In-vitro cultures were then set up to expose non-activated and activated monocytes to R5-, X4-, and dual (R5/X4)-tropic viruses; and the amount of HIV present on the cells was assayed. Results: Non-HIV-infected monocytes, after LPS stimulation, were confirmed to have an activated phenotype with increase in CD16 and CD69 surface expressions (p <0.05). The activation phenotype was supported by increase in TNFα production, p <0.05. The activated monocytes had increased surface CCR5 (from 21% to 98%; p = 0.05); and were found to have more R5-tropic virus than non-activated monocytes (p <0.05). Conclusions: Following activation by LPS, non-HIV-infected monocytes were found to have increase in surface CCR5. These activated monocytes, when exposed to R5-tropic virus, were found to have more virus compared to non-activated monocytes. The significance of the findings could lie in explaining how microbial translocation plays a role in HIV progression; and possibly promoting CCR5-directed strategies in treating HIV.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalImmunology and Immunogenetics Insights
Volume1
Issue number1
StatePublished - 2009
Externally publishedYes

Fingerprint

Monocytes
HIV
Phenotype
Lipopolysaccharides
Viruses
Flow Cytometry
Acquired Immunodeficiency Syndrome

Keywords

  • Activation
  • Dementia
  • Human immunodeficiency virus
  • Monocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Munsaka, S. M., Agsalda, M., Troelstrup, D., Hu, N., Yu, A., & Shiramizu, B. (2009). Characteristics of activated monocyte phenotype support R5-tropic human immunodeficiency virus. Immunology and Immunogenetics Insights, 1(1), 15-20.

Characteristics of activated monocyte phenotype support R5-tropic human immunodeficiency virus. / Munsaka, Sody M.; Agsalda, Melissa; Troelstrup, David; Hu, Ningjie; Yu, Andy; Shiramizu, Bruce.

In: Immunology and Immunogenetics Insights, Vol. 1, No. 1, 2009, p. 15-20.

Research output: Contribution to journalArticle

Munsaka, SM, Agsalda, M, Troelstrup, D, Hu, N, Yu, A & Shiramizu, B 2009, 'Characteristics of activated monocyte phenotype support R5-tropic human immunodeficiency virus', Immunology and Immunogenetics Insights, vol. 1, no. 1, pp. 15-20.
Munsaka, Sody M. ; Agsalda, Melissa ; Troelstrup, David ; Hu, Ningjie ; Yu, Andy ; Shiramizu, Bruce. / Characteristics of activated monocyte phenotype support R5-tropic human immunodeficiency virus. In: Immunology and Immunogenetics Insights. 2009 ; Vol. 1, No. 1. pp. 15-20.
@article{2137e945bcc04680b2c592474d1376fe,
title = "Characteristics of activated monocyte phenotype support R5-tropic human immunodeficiency virus",
abstract = "Background: Microbial translocation has been recognized as an important factor in monocyte activation and contributing to AIDS pathogenesis with elevated plasma lipopolysaccharide (LPS) levels, as a marker for microbial translocation, seen in advanced HIV disease. Therefore, the current study was undertaken to assess monocyte activation in vitro by LPS and to determine its impact on monocyte phenotype. Methods: Monocytes from non-HIV-infected donors were analyzed for CD14, CD16, CD69, TNFα, and CCR5 by flow cytometry pre- and post-stimulation with LPS. In-vitro cultures were then set up to expose non-activated and activated monocytes to R5-, X4-, and dual (R5/X4)-tropic viruses; and the amount of HIV present on the cells was assayed. Results: Non-HIV-infected monocytes, after LPS stimulation, were confirmed to have an activated phenotype with increase in CD16 and CD69 surface expressions (p <0.05). The activation phenotype was supported by increase in TNFα production, p <0.05. The activated monocytes had increased surface CCR5 (from 21{\%} to 98{\%}; p = 0.05); and were found to have more R5-tropic virus than non-activated monocytes (p <0.05). Conclusions: Following activation by LPS, non-HIV-infected monocytes were found to have increase in surface CCR5. These activated monocytes, when exposed to R5-tropic virus, were found to have more virus compared to non-activated monocytes. The significance of the findings could lie in explaining how microbial translocation plays a role in HIV progression; and possibly promoting CCR5-directed strategies in treating HIV.",
keywords = "Activation, Dementia, Human immunodeficiency virus, Monocytes",
author = "Munsaka, {Sody M.} and Melissa Agsalda and David Troelstrup and Ningjie Hu and Andy Yu and Bruce Shiramizu",
year = "2009",
language = "English (US)",
volume = "1",
pages = "15--20",
journal = "Immunology and Immunogenetics Insights",
issn = "1178-6345",
publisher = "Libertas Academica Ltd.",
number = "1",

}

TY - JOUR

T1 - Characteristics of activated monocyte phenotype support R5-tropic human immunodeficiency virus

AU - Munsaka, Sody M.

AU - Agsalda, Melissa

AU - Troelstrup, David

AU - Hu, Ningjie

AU - Yu, Andy

AU - Shiramizu, Bruce

PY - 2009

Y1 - 2009

N2 - Background: Microbial translocation has been recognized as an important factor in monocyte activation and contributing to AIDS pathogenesis with elevated plasma lipopolysaccharide (LPS) levels, as a marker for microbial translocation, seen in advanced HIV disease. Therefore, the current study was undertaken to assess monocyte activation in vitro by LPS and to determine its impact on monocyte phenotype. Methods: Monocytes from non-HIV-infected donors were analyzed for CD14, CD16, CD69, TNFα, and CCR5 by flow cytometry pre- and post-stimulation with LPS. In-vitro cultures were then set up to expose non-activated and activated monocytes to R5-, X4-, and dual (R5/X4)-tropic viruses; and the amount of HIV present on the cells was assayed. Results: Non-HIV-infected monocytes, after LPS stimulation, were confirmed to have an activated phenotype with increase in CD16 and CD69 surface expressions (p <0.05). The activation phenotype was supported by increase in TNFα production, p <0.05. The activated monocytes had increased surface CCR5 (from 21% to 98%; p = 0.05); and were found to have more R5-tropic virus than non-activated monocytes (p <0.05). Conclusions: Following activation by LPS, non-HIV-infected monocytes were found to have increase in surface CCR5. These activated monocytes, when exposed to R5-tropic virus, were found to have more virus compared to non-activated monocytes. The significance of the findings could lie in explaining how microbial translocation plays a role in HIV progression; and possibly promoting CCR5-directed strategies in treating HIV.

AB - Background: Microbial translocation has been recognized as an important factor in monocyte activation and contributing to AIDS pathogenesis with elevated plasma lipopolysaccharide (LPS) levels, as a marker for microbial translocation, seen in advanced HIV disease. Therefore, the current study was undertaken to assess monocyte activation in vitro by LPS and to determine its impact on monocyte phenotype. Methods: Monocytes from non-HIV-infected donors were analyzed for CD14, CD16, CD69, TNFα, and CCR5 by flow cytometry pre- and post-stimulation with LPS. In-vitro cultures were then set up to expose non-activated and activated monocytes to R5-, X4-, and dual (R5/X4)-tropic viruses; and the amount of HIV present on the cells was assayed. Results: Non-HIV-infected monocytes, after LPS stimulation, were confirmed to have an activated phenotype with increase in CD16 and CD69 surface expressions (p <0.05). The activation phenotype was supported by increase in TNFα production, p <0.05. The activated monocytes had increased surface CCR5 (from 21% to 98%; p = 0.05); and were found to have more R5-tropic virus than non-activated monocytes (p <0.05). Conclusions: Following activation by LPS, non-HIV-infected monocytes were found to have increase in surface CCR5. These activated monocytes, when exposed to R5-tropic virus, were found to have more virus compared to non-activated monocytes. The significance of the findings could lie in explaining how microbial translocation plays a role in HIV progression; and possibly promoting CCR5-directed strategies in treating HIV.

KW - Activation

KW - Dementia

KW - Human immunodeficiency virus

KW - Monocytes

UR - http://www.scopus.com/inward/record.url?scp=79251605975&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251605975&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:79251605975

VL - 1

SP - 15

EP - 20

JO - Immunology and Immunogenetics Insights

JF - Immunology and Immunogenetics Insights

SN - 1178-6345

IS - 1

ER -