Characteristics of wave fronts during ventricular fibrillation in human hearts with dilated cardiomyopathy: Role of increased fibrosis in the generation of reentry

Tsu Juey Wu, James J.C. Ong, Chun Hwang, John J. Lee, Michael C. Fishbein, Lawrence Czer, Alfredo Trento, Carlos Blanche, Robert M. Kass, William J. Mandel, Hrayr S. Karagueuzian, Peng Sheng Chen

Research output: Contribution to journalArticlepeer-review

131 Scopus citations

Abstract

Objectives. We sought to evaluate the characteristics of wave fronts during ventricular fibrillation (VF) in human hearts with dilated cardiomyopathy (DCM) and to determine the role of increased fibrosis in the generation of reentry during VF. Background. The role of increased fibrosis in reentry formation during human VF is unclear. Methods. Five hearts from transplant recipients with DCM were supported by Langendorff perfusion and were mapped during VF. A plaque electrode array with 477 bipolar electrodes (1.6-mm resolution) was used for epicardial mapping. In heart no. 5, we also used 440 transmural bipolar recordings. Each mapped area was analyzed histologically. Results. Fifteen runs of VF (8 s/run) recorded from the epicardium were analyzed, and 55 episodes of reentry were observed. The life span of reentry was short (one to four cycles), and the mean cycle length was 172 ± 24 ms. In heart no. 5, transmural scroll waves were demonstrated. The most common mode of initiation of reentry was epicardial breakthrough, followed by a line of conduction block parallel to the epicardial fiber orientation (34 [62%] of 55 episodes). In the areas with lines of block, histologic examination showed significant fibrosis separating the epicardial muscle fibers and bundles along the longitudinal axis of fiber orientation. The mean percent fibrous tissue in these areas (n = 20) was significantly higher than that in the areas without block (n = 28) (24 ± 7.5% vs. 10 ± 3.8%, p < 0.0001). Conclusions. In human hearts with DCM, epicardial reentrant wave fronts and transmural scroll waves were present during VF. Increased fibrosis provides a site for conduction block, leading to the continuous generation of reentry.

Original languageEnglish (US)
Pages (from-to)187-196
Number of pages10
JournalJournal of the American College of Cardiology
Volume32
Issue number1
DOIs
StatePublished - Jul 1998

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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