Characterization of a broad-based mosquito yeast interfering RNA larvicide with a conserved target site in mosquito semaphorin-1a genes

Keshava Mysore, Ping Li, Chien Wei Wang, Limb K. Hapairai, Nicholas D. Scheel, Jacob S. Realey, Longhua Sun, David W. Severson, Na Wei, Molly Scheel

Research output: Contribution to journalArticle

Abstract

Background: RNA interference (RNAi), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. Saccharomyces cerevisiae (baker's yeast) may represent an effective interfering RNA expression system that could be used directly for delivery of RNA pesticides to mosquito larvae. Here we describe characterization of a yeast larvicide developed through bioengineering of S. cerevisiae to express a short hairpin RNA (shRNA) targeting a conserved site in mosquito sema1a genes. Results: Experiments conducted on Aedes aegypti larvae demonstrated that the yeast larvicide effectively silences sema1a expression, generates severe neural defects, and induces high levels of larval mortality in laboratory, simulated-field, and semi-field experiments. The larvicide was also found to induce high levels of Aedes albopictus, Anopheles gambiae and Culex quinquefasciatus mortality. Conclusions: The results of these studies indicate that use of yeast interfering RNA larvicides targeting mosquito sema1a genes may represent a new biorational tool for mosquito control.

Original languageEnglish (US)
Article number256
JournalParasites and Vectors
Volume12
Issue number1
DOIs
StatePublished - May 22 2019

Fingerprint

Semaphorins
Culicidae
Yeasts
RNA
Saccharomyces cerevisiae
Aedes
Genes
Larva
Mosquito Control
Anopheles gambiae
Bioengineering
Culex
Mortality
RNA Interference
Pesticides
Small Interfering RNA

Keywords

  • Aedes aegypti
  • Anopheles gambiae
  • Arbovirus
  • Culex quinquefasciatus
  • Dengue
  • Insecticide
  • Malaria
  • Nervous system
  • RNA interference
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Characterization of a broad-based mosquito yeast interfering RNA larvicide with a conserved target site in mosquito semaphorin-1a genes. / Mysore, Keshava; Li, Ping; Wang, Chien Wei; Hapairai, Limb K.; Scheel, Nicholas D.; Realey, Jacob S.; Sun, Longhua; Severson, David W.; Wei, Na; Scheel, Molly.

In: Parasites and Vectors, Vol. 12, No. 1, 256, 22.05.2019.

Research output: Contribution to journalArticle

Mysore, Keshava ; Li, Ping ; Wang, Chien Wei ; Hapairai, Limb K. ; Scheel, Nicholas D. ; Realey, Jacob S. ; Sun, Longhua ; Severson, David W. ; Wei, Na ; Scheel, Molly. / Characterization of a broad-based mosquito yeast interfering RNA larvicide with a conserved target site in mosquito semaphorin-1a genes. In: Parasites and Vectors. 2019 ; Vol. 12, No. 1.
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