Characterization of a cDNA encoding murine coagulation factor VII

Esohe Idusogie, Elliot Rosen, Jie Ping Geng, Peter Carmeliet, Desiré Collen, Francis J. Castellino

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a h Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5'-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3' poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.

Original languageEnglish (US)
Pages (from-to)481-487
Number of pages7
JournalThrombosis and Haemostasis
Volume75
Issue number3
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

Factor VII
Nucleotides
Complementary DNA
Messenger RNA
Initiator Codon
Terminator Codon
Macaca mulatta
Gene Library
Open Reading Frames
Canidae
Rabbits
Amino Acids
Liver
Proteins

ASJC Scopus subject areas

  • Hematology

Cite this

Idusogie, E., Rosen, E., Geng, J. P., Carmeliet, P., Collen, D., & Castellino, F. J. (1996). Characterization of a cDNA encoding murine coagulation factor VII. Thrombosis and Haemostasis, 75(3), 481-487.

Characterization of a cDNA encoding murine coagulation factor VII. / Idusogie, Esohe; Rosen, Elliot; Geng, Jie Ping; Carmeliet, Peter; Collen, Desiré; Castellino, Francis J.

In: Thrombosis and Haemostasis, Vol. 75, No. 3, 03.1996, p. 481-487.

Research output: Contribution to journalArticle

Idusogie, E, Rosen, E, Geng, JP, Carmeliet, P, Collen, D & Castellino, FJ 1996, 'Characterization of a cDNA encoding murine coagulation factor VII', Thrombosis and Haemostasis, vol. 75, no. 3, pp. 481-487.
Idusogie E, Rosen E, Geng JP, Carmeliet P, Collen D, Castellino FJ. Characterization of a cDNA encoding murine coagulation factor VII. Thrombosis and Haemostasis. 1996 Mar;75(3):481-487.
Idusogie, Esohe ; Rosen, Elliot ; Geng, Jie Ping ; Carmeliet, Peter ; Collen, Desiré ; Castellino, Francis J. / Characterization of a cDNA encoding murine coagulation factor VII. In: Thrombosis and Haemostasis. 1996 ; Vol. 75, No. 3. pp. 481-487.
@article{7498e9a734534a4eb2cf00e64a836b02,
title = "Characterization of a cDNA encoding murine coagulation factor VII",
abstract = "The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a h Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5'-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3' poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.",
author = "Esohe Idusogie and Elliot Rosen and Geng, {Jie Ping} and Peter Carmeliet and Desir{\'e} Collen and Castellino, {Francis J.}",
year = "1996",
month = "3",
language = "English (US)",
volume = "75",
pages = "481--487",
journal = "Thrombosis and Haemostasis",
issn = "0340-6245",
publisher = "Schattauer GmbH",
number = "3",

}

TY - JOUR

T1 - Characterization of a cDNA encoding murine coagulation factor VII

AU - Idusogie, Esohe

AU - Rosen, Elliot

AU - Geng, Jie Ping

AU - Carmeliet, Peter

AU - Collen, Desiré

AU - Castellino, Francis J.

PY - 1996/3

Y1 - 1996/3

N2 - The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a h Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5'-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3' poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.

AB - The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a h Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5'-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3' poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.

UR - http://www.scopus.com/inward/record.url?scp=0029914396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029914396&partnerID=8YFLogxK

M3 - Article

C2 - 8701412

AN - SCOPUS:0029914396

VL - 75

SP - 481

EP - 487

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 3

ER -