Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption

R. O C Oreffo, G. J. Marshall, M. Kirchen, C. Garcia, W. E. Gallwitz, J. Chavez, G. R. Mundy, Lynda Bonewald

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Giant cell tumors of bone are common but unusual tumors that are comprised of multiple cell types. Most attention has been focused on the giant cells, which resemble osteoclasts morphologically and functionally. This study examines the properties of a cell line derived from mononuclear cells from one of these tumors, since it appears likely that these cells may be able to influence the activities of cells with the osteoclast phenotype. This cell line, C433, has the following characteristics: (1) it represents undifferentiated cells, not recognized by any known antigenic markers for leukocytes; (2) it contains tartrate-resistant acid phosphatase; (3) it responds to the osteotropic factors 1,25 dihydroxy-vitamin D3, insulin-like growth factor I and II, but not to parathyroid hormone; (4) it forms sarcomas in nude mice; and (5) it produces an activity that stimulates isolated avian and rat osteoclasts to resorb bone. This cell line may be useful in examining interactions between osteoclasts and accessory cells involved in bone resorption.

Original languageEnglish (US)
Pages (from-to)229-241
Number of pages13
JournalClinical Orthopaedics and Related Research
Issue number296
StatePublished - 1993
Externally publishedYes

Fingerprint

Giant Cell Tumors
Bone Resorption
Osteoclasts
Cell Line
Giant Cell Tumor of Bone
Insulin-Like Growth Factor II
Giant Cells
Parathyroid Hormone
Insulin-Like Growth Factor I
Nude Mice
Sarcoma
Neoplasms
Leukocytes
Phenotype
Bone and Bones

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Oreffo, R. O. C., Marshall, G. J., Kirchen, M., Garcia, C., Gallwitz, W. E., Chavez, J., ... Bonewald, L. (1993). Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption. Clinical Orthopaedics and Related Research, (296), 229-241.

Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption. / Oreffo, R. O C; Marshall, G. J.; Kirchen, M.; Garcia, C.; Gallwitz, W. E.; Chavez, J.; Mundy, G. R.; Bonewald, Lynda.

In: Clinical Orthopaedics and Related Research, No. 296, 1993, p. 229-241.

Research output: Contribution to journalArticle

Oreffo, ROC, Marshall, GJ, Kirchen, M, Garcia, C, Gallwitz, WE, Chavez, J, Mundy, GR & Bonewald, L 1993, 'Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption', Clinical Orthopaedics and Related Research, no. 296, pp. 229-241.
Oreffo, R. O C ; Marshall, G. J. ; Kirchen, M. ; Garcia, C. ; Gallwitz, W. E. ; Chavez, J. ; Mundy, G. R. ; Bonewald, Lynda. / Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption. In: Clinical Orthopaedics and Related Research. 1993 ; No. 296. pp. 229-241.
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