Characterization of a cell line, NKL, derived from an aggressive human natural killer cell leukemia

Michael Robertson, K. J. Cochran, C. Cameron, J. M. Le, R. Tantravahi, J. Ritz

Research output: Contribution to journalArticle

288 Citations (Scopus)

Abstract

Cell line NKL was established from the peripheral blood of a patient with CD3-CD16+CD56+ large granular lymphocyte (LGL) leukemia. The neoplastic LGL of this patient mediated natural killing and antibody-dependent cellular cytotoxicity (ADCC) and exhibited proliferative responses similar to normal CD16+CD56(dim) natural killer (NK) cells. The morphology of NKL cells resembles that of normal activated NK cells. The karyotype of NKL is 47, XY, add (1) (q42), +6, del (6) (q15 q23), del (17) (p11). NKL cells express CD2, CD6, CD11a, CD26, C27, CD29, CD38, CD43, CD58, CD81, CD94, CD95, class II MHC, and the C1.7.1 antigen, but do not express detectable levels of CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD28, α/β or γ/δ T cell receptors on the cell surface. The density of the CD16, CD56, and CD57 antigens declined markedly on NKL cells during prolonged in vitro culture. Nevertheless, NKL cells can mediate ADCC as well as natural killing. NKL cells are strictly dependent on interleukin-2 (IL-2) for sustained growth and die if deprived of IL-2 for more than 7 days. NKL cells proliferate in response to concentrations of IL-2 as low as 1 pM, but an optimal proliferative response requires ~100 pM IL-2. NKL cells growing in the presence of IL-2 express abundant IL-2R α with little or no detectable IL-2 β or γ chain on the cell surface; NKL cells deprived of IL-2 express high levels of both IL-2R α and β. IL-4, IL-7, and IL-12, unlike IL-2, do not maintain the viability of NKL cells. Furthermore, IL-1, IL-4, IL-6, IL-7, IL-12, tumor necrosis factor-α (TNF-α), interferon-α (IFN-α), and IFN-γ do not support the growth of NKL cells. The NKL cell line may prove useful for studies of human NK cell biology.

Original languageEnglish (US)
Pages (from-to)406-415
Number of pages10
JournalExperimental Hematology
Volume24
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Large Granular Lymphocytic Leukemia
Interleukin-2
Cell Line
Natural Killer Cells
Interleukin-7
Interleukin-12
Interleukin-4
Interferons
CD56 Antigens
CD57 Antigens
IgG Receptors
Antibodies
Growth
T-Cell Antigen Receptor
Karyotype
Interleukin-1
Cell Biology
Interleukin-6

Keywords

  • IL-2
  • Large granular lymphocyte leukemia
  • Natural killer cell
  • Natural killing

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Robertson, M., Cochran, K. J., Cameron, C., Le, J. M., Tantravahi, R., & Ritz, J. (1996). Characterization of a cell line, NKL, derived from an aggressive human natural killer cell leukemia. Experimental Hematology, 24(3), 406-415.

Characterization of a cell line, NKL, derived from an aggressive human natural killer cell leukemia. / Robertson, Michael; Cochran, K. J.; Cameron, C.; Le, J. M.; Tantravahi, R.; Ritz, J.

In: Experimental Hematology, Vol. 24, No. 3, 1996, p. 406-415.

Research output: Contribution to journalArticle

Robertson, M, Cochran, KJ, Cameron, C, Le, JM, Tantravahi, R & Ritz, J 1996, 'Characterization of a cell line, NKL, derived from an aggressive human natural killer cell leukemia', Experimental Hematology, vol. 24, no. 3, pp. 406-415.
Robertson, Michael ; Cochran, K. J. ; Cameron, C. ; Le, J. M. ; Tantravahi, R. ; Ritz, J. / Characterization of a cell line, NKL, derived from an aggressive human natural killer cell leukemia. In: Experimental Hematology. 1996 ; Vol. 24, No. 3. pp. 406-415.
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AU - Robertson, Michael

AU - Cochran, K. J.

AU - Cameron, C.

AU - Le, J. M.

AU - Tantravahi, R.

AU - Ritz, J.

PY - 1996

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N2 - Cell line NKL was established from the peripheral blood of a patient with CD3-CD16+CD56+ large granular lymphocyte (LGL) leukemia. The neoplastic LGL of this patient mediated natural killing and antibody-dependent cellular cytotoxicity (ADCC) and exhibited proliferative responses similar to normal CD16+CD56(dim) natural killer (NK) cells. The morphology of NKL cells resembles that of normal activated NK cells. The karyotype of NKL is 47, XY, add (1) (q42), +6, del (6) (q15 q23), del (17) (p11). NKL cells express CD2, CD6, CD11a, CD26, C27, CD29, CD38, CD43, CD58, CD81, CD94, CD95, class II MHC, and the C1.7.1 antigen, but do not express detectable levels of CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD28, α/β or γ/δ T cell receptors on the cell surface. The density of the CD16, CD56, and CD57 antigens declined markedly on NKL cells during prolonged in vitro culture. Nevertheless, NKL cells can mediate ADCC as well as natural killing. NKL cells are strictly dependent on interleukin-2 (IL-2) for sustained growth and die if deprived of IL-2 for more than 7 days. NKL cells proliferate in response to concentrations of IL-2 as low as 1 pM, but an optimal proliferative response requires ~100 pM IL-2. NKL cells growing in the presence of IL-2 express abundant IL-2R α with little or no detectable IL-2 β or γ chain on the cell surface; NKL cells deprived of IL-2 express high levels of both IL-2R α and β. IL-4, IL-7, and IL-12, unlike IL-2, do not maintain the viability of NKL cells. Furthermore, IL-1, IL-4, IL-6, IL-7, IL-12, tumor necrosis factor-α (TNF-α), interferon-α (IFN-α), and IFN-γ do not support the growth of NKL cells. The NKL cell line may prove useful for studies of human NK cell biology.

AB - Cell line NKL was established from the peripheral blood of a patient with CD3-CD16+CD56+ large granular lymphocyte (LGL) leukemia. The neoplastic LGL of this patient mediated natural killing and antibody-dependent cellular cytotoxicity (ADCC) and exhibited proliferative responses similar to normal CD16+CD56(dim) natural killer (NK) cells. The morphology of NKL cells resembles that of normal activated NK cells. The karyotype of NKL is 47, XY, add (1) (q42), +6, del (6) (q15 q23), del (17) (p11). NKL cells express CD2, CD6, CD11a, CD26, C27, CD29, CD38, CD43, CD58, CD81, CD94, CD95, class II MHC, and the C1.7.1 antigen, but do not express detectable levels of CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD28, α/β or γ/δ T cell receptors on the cell surface. The density of the CD16, CD56, and CD57 antigens declined markedly on NKL cells during prolonged in vitro culture. Nevertheless, NKL cells can mediate ADCC as well as natural killing. NKL cells are strictly dependent on interleukin-2 (IL-2) for sustained growth and die if deprived of IL-2 for more than 7 days. NKL cells proliferate in response to concentrations of IL-2 as low as 1 pM, but an optimal proliferative response requires ~100 pM IL-2. NKL cells growing in the presence of IL-2 express abundant IL-2R α with little or no detectable IL-2 β or γ chain on the cell surface; NKL cells deprived of IL-2 express high levels of both IL-2R α and β. IL-4, IL-7, and IL-12, unlike IL-2, do not maintain the viability of NKL cells. Furthermore, IL-1, IL-4, IL-6, IL-7, IL-12, tumor necrosis factor-α (TNF-α), interferon-α (IFN-α), and IFN-γ do not support the growth of NKL cells. The NKL cell line may prove useful for studies of human NK cell biology.

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