Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia: A model for oncogene regulation and late B-cell differentiation

William Harvey, Edward F. Srour, Rudolph Turner, Roger Carey, Rodney Maze, Bradley Starrett, Ravi Kanagala, Daniel Pereira, Peggy Merchant, Milton Taylor, Jan Jansen

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34 Scopus citations

Abstract

A B-lymphoblastoid cell line ESKOL, composed of differentiated cells resembling hairy-cell leukemia (HCL) has been established from the peripheral blood (PB) of a HCL patient. Morphologically, ESKOL cells share several features with HCL B cells. Flow cytometric analysis revealed that ESKOL cells express HC2M, CD21, PCA-1, CD24, FMC7, and CD25. Analysis by Northern-blot hybridization indicated that cultured cells expressed the oncogenes c-myc, H-ras and c-fos. RNA from 3T3 cells transfected with ESKOL. DNA hybridized with H-ras and c-fos DNA probes. The ESKOL cells cultured in the presence of increasing concentrations, of alpha interferon demonstrated a decrease in the rate of cellular growth and an increase in the expression of CD21, CD25, FMC7 and PCA-1. Scanning electron microscopy revealed that cells incubated in the presence of alpha interferon underwent membranous changes with a loss of villosity. These observations suggest that IFN tends to drive HC out of their developmental arrest towards maturation.

Original languageEnglish (US)
Pages (from-to)733-744
Number of pages12
JournalLeukemia Research
Volume15
Issue number8
DOIs
StatePublished - 1991

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Keywords

  • c-fos
  • c-myc
  • H-ras
  • hairy-cell leukemia
  • Interferon
  • oncogenes

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Harvey, W., Srour, E. F., Turner, R., Carey, R., Maze, R., Starrett, B., Kanagala, R., Pereira, D., Merchant, P., Taylor, M., & Jansen, J. (1991). Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia: A model for oncogene regulation and late B-cell differentiation. Leukemia Research, 15(8), 733-744. https://doi.org/10.1016/0145-2126(91)90077-7