Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia: A model for oncogene regulation and late B-cell differentiation

William Harvey, Edward Srour, Rudolph Turner, Roger Carey, Rodney Maze, Bradley Starrett, Ravi Kanagala, Daniel Pereira, Peggy Merchant, Milton Taylor, Jan Jansen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A B-lymphoblastoid cell line ESKOL, composed of differentiated cells resembling hairy-cell leukemia (HCL) has been established from the peripheral blood (PB) of a HCL patient. Morphologically, ESKOL cells share several features with HCL B cells. Flow cytometric analysis revealed that ESKOL cells express HC2M, CD21, PCA-1, CD24, FMC7, and CD25. Analysis by Northern-blot hybridization indicated that cultured cells expressed the oncogenes c-myc, H-ras and c-fos. RNA from 3T3 cells transfected with ESKOL. DNA hybridized with H-ras and c-fos DNA probes. The ESKOL cells cultured in the presence of increasing concentrations, of alpha interferon demonstrated a decrease in the rate of cellular growth and an increase in the expression of CD21, CD25, FMC7 and PCA-1. Scanning electron microscopy revealed that cells incubated in the presence of alpha interferon underwent membranous changes with a loss of villosity. These observations suggest that IFN tends to drive HC out of their developmental arrest towards maturation.

Original languageEnglish
Pages (from-to)733-744
Number of pages12
JournalLeukemia Research
Volume15
Issue number8
DOIs
StatePublished - 1991

Fingerprint

Hairy Cell Leukemia
Oncogenes
Cell Differentiation
B-Lymphocytes
Cell Line
Passive Cutaneous Anaphylaxis
Interferon-alpha
Cultured Cells
3T3 Cells
myc Genes
DNA Probes
Northern Blotting
Electron Scanning Microscopy
RNA
DNA
Growth

Keywords

  • c-fos
  • c-myc
  • H-ras
  • hairy-cell leukemia
  • Interferon
  • oncogenes

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia : A model for oncogene regulation and late B-cell differentiation. / Harvey, William; Srour, Edward; Turner, Rudolph; Carey, Roger; Maze, Rodney; Starrett, Bradley; Kanagala, Ravi; Pereira, Daniel; Merchant, Peggy; Taylor, Milton; Jansen, Jan.

In: Leukemia Research, Vol. 15, No. 8, 1991, p. 733-744.

Research output: Contribution to journalArticle

Harvey, W, Srour, E, Turner, R, Carey, R, Maze, R, Starrett, B, Kanagala, R, Pereira, D, Merchant, P, Taylor, M & Jansen, J 1991, 'Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia: A model for oncogene regulation and late B-cell differentiation', Leukemia Research, vol. 15, no. 8, pp. 733-744. https://doi.org/10.1016/0145-2126(91)90077-7
Harvey, William ; Srour, Edward ; Turner, Rudolph ; Carey, Roger ; Maze, Rodney ; Starrett, Bradley ; Kanagala, Ravi ; Pereira, Daniel ; Merchant, Peggy ; Taylor, Milton ; Jansen, Jan. / Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia : A model for oncogene regulation and late B-cell differentiation. In: Leukemia Research. 1991 ; Vol. 15, No. 8. pp. 733-744.
@article{2447287cf43f49fbbf047befb4053c52,
title = "Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia: A model for oncogene regulation and late B-cell differentiation",
abstract = "A B-lymphoblastoid cell line ESKOL, composed of differentiated cells resembling hairy-cell leukemia (HCL) has been established from the peripheral blood (PB) of a HCL patient. Morphologically, ESKOL cells share several features with HCL B cells. Flow cytometric analysis revealed that ESKOL cells express HC2M, CD21, PCA-1, CD24, FMC7, and CD25. Analysis by Northern-blot hybridization indicated that cultured cells expressed the oncogenes c-myc, H-ras and c-fos. RNA from 3T3 cells transfected with ESKOL. DNA hybridized with H-ras and c-fos DNA probes. The ESKOL cells cultured in the presence of increasing concentrations, of alpha interferon demonstrated a decrease in the rate of cellular growth and an increase in the expression of CD21, CD25, FMC7 and PCA-1. Scanning electron microscopy revealed that cells incubated in the presence of alpha interferon underwent membranous changes with a loss of villosity. These observations suggest that IFN tends to drive HC out of their developmental arrest towards maturation.",
keywords = "c-fos, c-myc, H-ras, hairy-cell leukemia, Interferon, oncogenes",
author = "William Harvey and Edward Srour and Rudolph Turner and Roger Carey and Rodney Maze and Bradley Starrett and Ravi Kanagala and Daniel Pereira and Peggy Merchant and Milton Taylor and Jan Jansen",
year = "1991",
doi = "10.1016/0145-2126(91)90077-7",
language = "English",
volume = "15",
pages = "733--744",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Limited",
number = "8",

}

TY - JOUR

T1 - Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia

T2 - A model for oncogene regulation and late B-cell differentiation

AU - Harvey, William

AU - Srour, Edward

AU - Turner, Rudolph

AU - Carey, Roger

AU - Maze, Rodney

AU - Starrett, Bradley

AU - Kanagala, Ravi

AU - Pereira, Daniel

AU - Merchant, Peggy

AU - Taylor, Milton

AU - Jansen, Jan

PY - 1991

Y1 - 1991

N2 - A B-lymphoblastoid cell line ESKOL, composed of differentiated cells resembling hairy-cell leukemia (HCL) has been established from the peripheral blood (PB) of a HCL patient. Morphologically, ESKOL cells share several features with HCL B cells. Flow cytometric analysis revealed that ESKOL cells express HC2M, CD21, PCA-1, CD24, FMC7, and CD25. Analysis by Northern-blot hybridization indicated that cultured cells expressed the oncogenes c-myc, H-ras and c-fos. RNA from 3T3 cells transfected with ESKOL. DNA hybridized with H-ras and c-fos DNA probes. The ESKOL cells cultured in the presence of increasing concentrations, of alpha interferon demonstrated a decrease in the rate of cellular growth and an increase in the expression of CD21, CD25, FMC7 and PCA-1. Scanning electron microscopy revealed that cells incubated in the presence of alpha interferon underwent membranous changes with a loss of villosity. These observations suggest that IFN tends to drive HC out of their developmental arrest towards maturation.

AB - A B-lymphoblastoid cell line ESKOL, composed of differentiated cells resembling hairy-cell leukemia (HCL) has been established from the peripheral blood (PB) of a HCL patient. Morphologically, ESKOL cells share several features with HCL B cells. Flow cytometric analysis revealed that ESKOL cells express HC2M, CD21, PCA-1, CD24, FMC7, and CD25. Analysis by Northern-blot hybridization indicated that cultured cells expressed the oncogenes c-myc, H-ras and c-fos. RNA from 3T3 cells transfected with ESKOL. DNA hybridized with H-ras and c-fos DNA probes. The ESKOL cells cultured in the presence of increasing concentrations, of alpha interferon demonstrated a decrease in the rate of cellular growth and an increase in the expression of CD21, CD25, FMC7 and PCA-1. Scanning electron microscopy revealed that cells incubated in the presence of alpha interferon underwent membranous changes with a loss of villosity. These observations suggest that IFN tends to drive HC out of their developmental arrest towards maturation.

KW - c-fos

KW - c-myc

KW - H-ras

KW - hairy-cell leukemia

KW - Interferon

KW - oncogenes

UR - http://www.scopus.com/inward/record.url?scp=0025737745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025737745&partnerID=8YFLogxK

U2 - 10.1016/0145-2126(91)90077-7

DO - 10.1016/0145-2126(91)90077-7

M3 - Article

C2 - 1895754

AN - SCOPUS:0025737745

VL - 15

SP - 733

EP - 744

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 8

ER -