Characterization of a novel polyclonal anti-hypusine antibody

Yurika Nishiki, Thomas B. Farb, Jessica Friedrich, Krister Bokvist, Raghavendra G. Mirmira, Bernhard Maier

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.

Original languageEnglish (US)
Article number421
Pages (from-to)1-5
Number of pages5
JournalSpringerPlus
Volume2
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Anti-Idiotypic Antibodies
Cell Proliferation
Amino Acids
Antibodies
Proteins
Therapeutics
hypusine

Keywords

  • Antibody
  • Cell lines
  • EIF5A
  • Hypusine
  • Recombinant proteins

ASJC Scopus subject areas

  • General

Cite this

Characterization of a novel polyclonal anti-hypusine antibody. / Nishiki, Yurika; Farb, Thomas B.; Friedrich, Jessica; Bokvist, Krister; Mirmira, Raghavendra G.; Maier, Bernhard.

In: SpringerPlus, Vol. 2, No. 1, 421, 01.01.2013, p. 1-5.

Research output: Contribution to journalArticle

Nishiki, Yurika ; Farb, Thomas B. ; Friedrich, Jessica ; Bokvist, Krister ; Mirmira, Raghavendra G. ; Maier, Bernhard. / Characterization of a novel polyclonal anti-hypusine antibody. In: SpringerPlus. 2013 ; Vol. 2, No. 1. pp. 1-5.
@article{3cdafa4e13a1459390c6c13c79d3a074,
title = "Characterization of a novel polyclonal anti-hypusine antibody",
abstract = "The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.",
keywords = "Antibody, Cell lines, EIF5A, Hypusine, Recombinant proteins",
author = "Yurika Nishiki and Farb, {Thomas B.} and Jessica Friedrich and Krister Bokvist and Mirmira, {Raghavendra G.} and Bernhard Maier",
year = "2013",
month = "1",
day = "1",
doi = "10.1186/2193-1801-2-421",
language = "English (US)",
volume = "2",
pages = "1--5",
journal = "SpringerPlus",
issn = "2193-1801",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "1",

}

TY - JOUR

T1 - Characterization of a novel polyclonal anti-hypusine antibody

AU - Nishiki, Yurika

AU - Farb, Thomas B.

AU - Friedrich, Jessica

AU - Bokvist, Krister

AU - Mirmira, Raghavendra G.

AU - Maier, Bernhard

PY - 2013/1/1

Y1 - 2013/1/1

N2 - The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.

AB - The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.

KW - Antibody

KW - Cell lines

KW - EIF5A

KW - Hypusine

KW - Recombinant proteins

UR - http://www.scopus.com/inward/record.url?scp=84887291453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887291453&partnerID=8YFLogxK

U2 - 10.1186/2193-1801-2-421

DO - 10.1186/2193-1801-2-421

M3 - Article

C2 - 24024105

AN - SCOPUS:84887291453

VL - 2

SP - 1

EP - 5

JO - SpringerPlus

JF - SpringerPlus

SN - 2193-1801

IS - 1

M1 - 421

ER -