Characterization of a novel polyclonal anti-hypusine antibody

Yurika Nishiki, Thomas B. Farb, Jessica Friedrich, Krister Bokvist, Raghavendra G. Mirmira, Bernhard Maier

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.

Original languageEnglish (US)
Article number421
Pages (from-to)1-5
Number of pages5
JournalSpringerPlus
Volume2
Issue number1
DOIs
StatePublished - Jan 1 2013

Keywords

  • Antibody
  • Cell lines
  • EIF5A
  • Hypusine
  • Recombinant proteins

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Characterization of a novel polyclonal anti-hypusine antibody'. Together they form a unique fingerprint.

  • Cite this