Characterization of a novel polyclonal anti-hypusine antibody

Yurika Nishiki, Thomas B. Farb, Jessica Friedrich, Krister Bokvist, Raghu Mirmira, Bernhard Maier

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalSpringerPlus
Volume2
Issue number1
DOIs
StatePublished - 2013

Fingerprint

Anti-Idiotypic Antibodies
Cell Proliferation
Amino Acids
Antibodies
Proteins
Therapeutics
hypusine

Keywords

  • Antibody
  • Cell lines
  • EIF5A
  • Hypusine
  • Recombinant proteins

ASJC Scopus subject areas

  • General

Cite this

Characterization of a novel polyclonal anti-hypusine antibody. / Nishiki, Yurika; Farb, Thomas B.; Friedrich, Jessica; Bokvist, Krister; Mirmira, Raghu; Maier, Bernhard.

In: SpringerPlus, Vol. 2, No. 1, 2013, p. 1-5.

Research output: Contribution to journalArticle

Nishiki, Yurika ; Farb, Thomas B. ; Friedrich, Jessica ; Bokvist, Krister ; Mirmira, Raghu ; Maier, Bernhard. / Characterization of a novel polyclonal anti-hypusine antibody. In: SpringerPlus. 2013 ; Vol. 2, No. 1. pp. 1-5.
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