Characterization of an isoelectric focusing variant of SAA1 (ASP-72) in a family of Turkish origin

Barbara Kluve-Beckerman, Ernst Malle, Herbert Vitt, Cathleen Pfeiffer, Merrill Benson, Armin Steinmetz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An acidic variant of serum amyloid A (SAA) identified previously by isoelectrofocusing in a family of Turkish origin has been characterized at the genomic level. DNA sequence analysis revealed that individuals expressing the variant pI6.1 pI5.7 isoforms (the mother and three of four children) were heterozygous at the SAA1 gene locus. Their SAA1 gene sequences contained an adenine, as well as the usual guanine, at the position corresponding to the second base of codon 72. The presence of both bases predicts two SAA1 protein sequences, one having aspartic acid and the other glycine at position 72. While the Gly-72 SAA1 (± Arg-1) sequence represents the normal pI6.5 pI6.0 isoforms, the Asp-72 SAA1 (± Arg-1) sequence corresponds to the variant pI6.1 pI5.7 isoforms.

Original languageEnglish
Pages (from-to)1097-1102
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume181
Issue number3
DOIs
StatePublished - Dec 31 1991

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Isoelectric Focusing
Protein Isoforms
Genes
Serum Amyloid A Protein
DNA sequences
Guanine
Adenine
DNA Sequence Analysis
Aspartic Acid
Codon
Glycine
Mothers
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterization of an isoelectric focusing variant of SAA1 (ASP-72) in a family of Turkish origin. / Kluve-Beckerman, Barbara; Malle, Ernst; Vitt, Herbert; Pfeiffer, Cathleen; Benson, Merrill; Steinmetz, Armin.

In: Biochemical and Biophysical Research Communications, Vol. 181, No. 3, 31.12.1991, p. 1097-1102.

Research output: Contribution to journalArticle

Kluve-Beckerman, Barbara ; Malle, Ernst ; Vitt, Herbert ; Pfeiffer, Cathleen ; Benson, Merrill ; Steinmetz, Armin. / Characterization of an isoelectric focusing variant of SAA1 (ASP-72) in a family of Turkish origin. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 181, No. 3. pp. 1097-1102.
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