Characterization of angiotensin-converting enzyme in the gills of rainbow trout, Salmo gairdneri (Richardson)

D. W. Lipke, R. L. Thomas, Kenneth Olson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Several physical and chemical parameters of angiotensin-converting enzyme (ACE) were determined using a spectrophotometric assay of gill tissue homogenates from rainbow trout. This assay is based on the evolution of free hippuric acid via enzymatic cleavage of histidyl-leucine from the synthetic substrate hippuryl-l-histidyl-l-leucine (HHL). Piscine ACE exhibited enzymatic and kinetic properties similar to those reported for the partially purified mammalian enzyme. Proteolytic activity was both temperature and pH dependent and demonstrated hyperbolic kinetics with an apparent Km of 2.5 mM. Hydrolysis of HHL was activated by Cl- at concentrations between 20 mM and 100 mM. Captopril (1 × 10-6 M) and MK-422 (1 × 10-6 M) blocked trout gill ACE activity, however, EDTA was inhibitory only at high concentrations (1 × 10-3 M). These results demonstrate that trout ACE is functionally similar to mammalian ACE and that the spectro-photometric assay for ACE developed by Cushman and Cheung can be applied to analysis of converting enzyme activity in fish tissue homogenates.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalFish Physiology and Biochemistry
Volume3
Issue number2
DOIs
StatePublished - Mar 1987

Fingerprint

Oncorhynchus mykiss
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
rainbow
gills
enzyme
Leucine
leucine
Assays
Trout
Enzyme activity
assay
trout
enzyme activity
assays
hippuric acid
Enalaprilat
Tissue
kinetics
Kinetics

Keywords

  • captopril
  • dipeptidyl carboxypeptidase
  • dipeptidyl hydrolase
  • fish
  • hippuryl-histidyl-leucine
  • MK-422
  • spectrophotometric assay

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Aquatic Science

Cite this

Characterization of angiotensin-converting enzyme in the gills of rainbow trout, Salmo gairdneri (Richardson). / Lipke, D. W.; Thomas, R. L.; Olson, Kenneth.

In: Fish Physiology and Biochemistry, Vol. 3, No. 2, 03.1987, p. 91-97.

Research output: Contribution to journalArticle

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