Characterization of cell lines derived from enlarged spleens induced in C57BL/6 mice by the variant BSB strain of Friend erythroleukemia virus

Roy W. Geib, Rita Anand, Frank Lilly

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

BSB is a variant strain of Friend vims selected for pathogenicity in C57BL/6 mice that are resistant to parental Friend virus strains by virtue of their homozygosity for the recessive Fv-2r allele (Steeves et al., 1970, Int. J. Cancer 5, 346-356). Lines and clones of erythroleukemia cells could readily be established in culture from the enlarged spleens of BSB-infected Fv-2r homozygotes. All lines expressed viral gene products and could be induced to express hemoglobin. Some lines produced infectious virus. In addition to the viral envelope-related proteins (gPr85, gp70, and gp52) detected by precipitation with goat anti-Rauscher gp70 antiserum from tumor cell lines induced by parental Friend virus strains, BSB-induced cell lines also expressed gp80, p52, and gp45 products precipitable with the same antiserum. A rat monoclonal antibody that recognizes an epitope of an aminoterminal region of gp52 (Wolff et al., 1982, J. Virol. 43, 472-481) also precipitated the gp80 and gp45 viral proteins. The data indicate that the BSB strain of Friend virus is oncogenic in Fu-2r homozygotes. Transformation is correlated with the expression of an altered SFFV env-gene product.

Original languageEnglish
Pages (from-to)61-72
Number of pages12
JournalVirus Research
Volume8
Issue number1
DOIs
StatePublished - 1987

Fingerprint

Friend murine leukemia virus
Leukemia, Erythroblastic, Acute
Splenomegaly
Inbred C57BL Mouse
Homozygote
Viral Proteins
Cell Line
Immune Sera
Spleen Focus-Forming Viruses
env Gene Products
Viral Envelope Proteins
Tumor Cell Line
Goats
Virulence
Epitopes
Hemoglobins
Clone Cells
Alleles
Monoclonal Antibodies
Viruses

Keywords

  • BSB strain
  • Friend virus
  • Mouse C57BL/6
  • Oncogenesis
  • Virus expression

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Characterization of cell lines derived from enlarged spleens induced in C57BL/6 mice by the variant BSB strain of Friend erythroleukemia virus. / Geib, Roy W.; Anand, Rita; Lilly, Frank.

In: Virus Research, Vol. 8, No. 1, 1987, p. 61-72.

Research output: Contribution to journalArticle

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AB - BSB is a variant strain of Friend vims selected for pathogenicity in C57BL/6 mice that are resistant to parental Friend virus strains by virtue of their homozygosity for the recessive Fv-2r allele (Steeves et al., 1970, Int. J. Cancer 5, 346-356). Lines and clones of erythroleukemia cells could readily be established in culture from the enlarged spleens of BSB-infected Fv-2r homozygotes. All lines expressed viral gene products and could be induced to express hemoglobin. Some lines produced infectious virus. In addition to the viral envelope-related proteins (gPr85, gp70, and gp52) detected by precipitation with goat anti-Rauscher gp70 antiserum from tumor cell lines induced by parental Friend virus strains, BSB-induced cell lines also expressed gp80, p52, and gp45 products precipitable with the same antiserum. A rat monoclonal antibody that recognizes an epitope of an aminoterminal region of gp52 (Wolff et al., 1982, J. Virol. 43, 472-481) also precipitated the gp80 and gp45 viral proteins. The data indicate that the BSB strain of Friend virus is oncogenic in Fu-2r homozygotes. Transformation is correlated with the expression of an altered SFFV env-gene product.

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