Characterization of chondrocytes from bovine articular cartilage. I. Metabolic and morphological experimental studies

Stephen Trippel, M. G. Ehrlich, L. Lippiello, H. J. Mankin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Articular chondrocytes were enzymatically isolated from mature bovine cartilage and separated by density zone-velocity sedimentation in a gradient. For cells from different levels of the gradient, size and staining characteristics were determined and rates of incorporation of tritiated cytidine and radiosulfate were measured as indicators of RNA and sulfated glycosaminoglycan synthesis, respectively. The data clearly show that the chondrocyte population is composed of cells that vary continuously in size and metabolic activity from one limit to another. The largest cells also demonstrated the greatest RNA production while the smallest cells had the least. There was, however, no such differentiation of sulfated proteoglycan production. The data reported here provide an essential baseline in terms of the variations in size and metabolic activity observed in normal chondrocyte populations. This information can be used to: (1) reveal changes in the characteristics of chondrocytes from diseased cartilage, and (2) identify subpopulations of chondrocytes with altered functions in normal or diseased articular or epiphyseal growth-plate cartilage when studying clinical problems related to cartilage.

Original languageEnglish (US)
Pages (from-to)816-820
Number of pages5
JournalJournal of Bone and Joint Surgery - Series A
Volume62
Issue number5
StatePublished - 1980
Externally publishedYes

Fingerprint

Articular Cartilage
Chondrocytes
Cartilage
Growth Plate
A73025
Joints
Cartilage Diseases
RNA
Cytidine
Proteoglycans
Population
Staining and Labeling

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Characterization of chondrocytes from bovine articular cartilage. I. Metabolic and morphological experimental studies. / Trippel, Stephen; Ehrlich, M. G.; Lippiello, L.; Mankin, H. J.

In: Journal of Bone and Joint Surgery - Series A, Vol. 62, No. 5, 1980, p. 816-820.

Research output: Contribution to journalArticle

Trippel, Stephen ; Ehrlich, M. G. ; Lippiello, L. ; Mankin, H. J. / Characterization of chondrocytes from bovine articular cartilage. I. Metabolic and morphological experimental studies. In: Journal of Bone and Joint Surgery - Series A. 1980 ; Vol. 62, No. 5. pp. 816-820.
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