Characterization of definitive lymphohematopoietic stem cells in the day 9 murine yolk sac

Mervin C. Yoder, Kelly Hiatt, Parmesh Dutt, Pinku Mukherjee, David M. Bodine, Donald Orlic

Research output: Contribution to journalArticle

325 Scopus citations

Abstract

The site of origin of lymphohematopoietic stem cells (HSC) that initiate definitive blood cell production in the murine fetal liver is controversial. Contrary to reports that the preliver yolk sac does not contain definitive HSC, we observed that CD34+ day 9 yolk sac cells repopulated multiple blood cell lineages in newborn hosts for at least 1 year. Furthermore, 100 CD34+c- Kit+ day 9 yolk sac or para-aortic splanchnopleura (P-Sp) cells, known to give rise to embryonic HSC, similarly repopulated hematopoiesis in recipient hosts. Surprisingly, 37-fold more CD34+c-Kit+ cells reside in the day 9 yolk sac than in the P-Sp. In sum, definitive HSC are coexistent, but not equal in number, in the murine yolk sac and P-Sp prior to fetal liver colonization.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalImmunity
Volume7
Issue number3
DOIs
StatePublished - Sep 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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