Characterization of Gac1p, a regulatory subunit of protein phosphatase type I involved in glycogen accumulation in Saccharomyces cerevisiae

X. Wu, H. Hart, C. Cheng, P. Roach, K. Tatchell

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

GAC1 and GLC7 encode regulatory and catalytic subunits, respectively, of a type 1 phosphatase (PP1) in Saccharomyces cerevisiae that controls glycogen synthesis by regulating the phosphorylation state of glycogen synthase (Gsy2p). To investigate the role of Gac1p in this process, a set of GAC1 deletions were tested for their ability to complement a gac1 null mutation and to associate with Glc7p and with Gsy2p. The N-terminal 93 amino acids of Gac1p are necessary and sufficient for the interaction with Glc7p, whereas a region spanning residues 130-502 is required for Gsy2p binding. Both domains are required for full activity in vivo, although the Glc7p-binding domain retains some residual activity and can alter the phosphorylase a phosphatase activity of Glc7p in vitro. Further mutational analysis showed that Val71 and Phe73 of Gac1p are necessary for binding to Glc7p, while Asn356 and Tyr357 of Gac1p are necessary for binding to Gsy2p. These results suggest that Gac1p targets PP1 to its substrate Gsy2p and that Gac1p may alter the catalytic activity of PP1. Our data also show that overexpression of Gac1p affects glucose repression and ion homeostasis, two additional targets of GLC7, suggesting that multiple regulatory subunits compete for Glc7p binding in vivo.

Original languageEnglish (US)
Pages (from-to)622-635
Number of pages14
JournalMolecular Genetics and Genomics
Volume265
Issue number4
DOIs
StatePublished - Aug 29 2001

Keywords

  • GAC1
  • GLC7
  • Glycogen synthase
  • Protein phosphatase
  • Yeast

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Characterization of Gac1p, a regulatory subunit of protein phosphatase type I involved in glycogen accumulation in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

  • Cite this