Characterization of gains, losses, and regional amplification in testicular germ cell tumor cell lines by comparative genomic hybridization

Octavian Henegariu, Nyla A. Heerema, Virginia Thurston, Sin Ho Jung, Martin Pera, Gail Vance

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Abstract

We have performed comparative genomic hybridization on 12 testicular germ cell tumor (TGCT) cell lines and one paraffin-embedded surgical specimen to identify and characterize genome-wide gains and losses of chromosomes in these specimens. All specimens demonstrated overrepresentation of 12p. Other significant chromosomal gains, apart from 12p, included the X chromosome and chromosome arms 1q and 20q. Chromosomal losses were observed for chromosomes 4 and 18 and chromosome arms 2q, 9q, and 13q. Genomic differences were observed between an embryonal carcinoma component of a mixed tumor, 833K, and its cisplastin-resistant derivative line, 64CP, including losses of 6q23∼qter and 9p22∼q21. Five lines also demonstrated gain of 12p and additional 12p12∼p13 material. Similarly, two lines demonstrated gain of 12p and additional 12p11.2∼p12 material. The data supports the consistent gain of 12p in adult TGCT cell lines and additional regional amplification of 12p in some lines. This regional amplification has been observed in both primary tumor specimens and TGCT cell lines and may support a hypothesis that at least two different regions of 12p, one proximal and one distal, harbor genes important for the pathogenesis of testicular germ cell neoplasia.

Original languageEnglish
Pages (from-to)14-20
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume148
Issue number1
DOIs
StatePublished - Jan 1 2004

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Comparative Genomic Hybridization
Tumor Cell Line
Chromosomes
Embryonal Carcinoma
Chromosomes, Human, Pair 18
Neoplasms
Chromosomes, Human, Pair 4
X Chromosome
Germ Cells
Paraffin
Genome
Genes
Testicular Germ Cell Tumor

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Characterization of gains, losses, and regional amplification in testicular germ cell tumor cell lines by comparative genomic hybridization. / Henegariu, Octavian; Heerema, Nyla A.; Thurston, Virginia; Jung, Sin Ho; Pera, Martin; Vance, Gail.

In: Cancer Genetics and Cytogenetics, Vol. 148, No. 1, 01.01.2004, p. 14-20.

Research output: Contribution to journalArticle

Henegariu, Octavian ; Heerema, Nyla A. ; Thurston, Virginia ; Jung, Sin Ho ; Pera, Martin ; Vance, Gail. / Characterization of gains, losses, and regional amplification in testicular germ cell tumor cell lines by comparative genomic hybridization. In: Cancer Genetics and Cytogenetics. 2004 ; Vol. 148, No. 1. pp. 14-20.
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