Characterization of Gallus domesticus α- N-acetyl-galactosaminidase blood group A2 activity

R. Phillips, T. Mawhinney, M. Harmata, Daniel Smith

Research output: Contribution to journalArticle

Abstract

Soluble A antigens and an enzyme-linked immunosorbent assay (ELISA) using type A2 erythrocyte membranes were used to study the activity of an α-N-acetyl-galactosaminidase from Gallus domesticus (domestic chicken). The enzyme readily hydrolyzed the terminal N-acetyl-α-D-galactosamine of the A antigen under a variety of conditions, converting it to H antigen. Conversion of the A antigen to H antigen produces blood type O, which is universally transfusable. These preliminary studies are important in determining optimal conditions for enzymatic conversion of blood type A to O if efficient large scale production of enzymatically converted, universally transfusable red blood cells is to be achieved.

Original languageEnglish (US)
Pages (from-to)63-79
Number of pages17
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume23
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Hexosaminidases
Antigens
Blood Group Antigens
varespladib methyl
Chickens
Blood
Acetylgalactosamine
Immunosorbents
Erythrocyte Membrane
Enzymes
Galactosamine
Assays
Erythrocytes
Enzyme-Linked Immunosorbent Assay
Cells
Membranes
H antigen

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

Cite this

Characterization of Gallus domesticus α- N-acetyl-galactosaminidase blood group A2 activity. / Phillips, R.; Mawhinney, T.; Harmata, M.; Smith, Daniel.

In: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, Vol. 23, No. 1, 1995, p. 63-79.

Research output: Contribution to journalArticle

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