Characterization of gallus domesticus a-n-acetyl-galactosaminidase blood group

A. Activity, Roy Phillips, Thomas Mawhinney, Michael Harmata, Daniel Smith

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Abstract

Soluble A antigens and an enzyme-linked immunosorbent assay (ELISA) using type A2 erythrocyte membranes were used to study the Activity of an a-N-acetyl-galactosaminidase from Gallus domesticus (domestic chicken). The enzyme readily hydrolyzed the terminal N-acetyl-oc-D-galactosamine of the A antigen under a variety of conditions, converting it to H antigen. Conversion of the A antigen to H antigen produces blood type O., which is universally transfusable. These preliminary studies are important in determining optimal conditions for enzymatic conversion of blood type A to O if efficient large scale production of enzymatically converted, universally transfusable red blood cells is to be achieved.

Original languageEnglish (US)
Pages (from-to)63-79
Number of pages17
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume23
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Biomedical Engineering
  • Biotechnology

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