Soluble A antigens and an enzyme-linked immunosorbent assay (ELISA) using type A2 erythrocyte membranes were used to study the Activity of an a-N-acetyl-galactosaminidase from Gallus domesticus (domestic chicken). The enzyme readily hydrolyzed the terminal N-acetyl-oc-D-galactosamine of the A antigen under a variety of conditions, converting it to H antigen. Conversion of the A antigen to H antigen produces blood type O., which is universally transfusable. These preliminary studies are important in determining optimal conditions for enzymatic conversion of blood type A to O if efficient large scale production of enzymatically converted, universally transfusable red blood cells is to be achieved.
|Original language||English (US)|
|Number of pages||17|
|Journal||Artificial Cells, Blood Substitutes, and Immobilization Biotechnology|
|State||Published - 1995|
ASJC Scopus subject areas
- Biomedical Engineering