Characterization of Growth Factor-Modulated Events Regulating Cellular Proliferation

Maureen A. Harrington, W. Jackson Pledger

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

This chapter various methods employing requirements for a specific growth factor(s) and essential nutrients are described that can be used to synchronize BALB/c 3T3 cells at the various regulatory points within G0/G1. Cells synchronized in this fashion can be used to (1) study the mechanism of action of certain growth factors at the specific cell cycle time when they are required and (2) delineate and order those cellular events required for G0/G1 traverse. Traverse of density-arrested fibroblastic cells through G0/GI is mediated by multiple growth factors with sequential or interdependent actions. Platelet-derived growth factor (PDGF) and other known competence factors initiate the proliferative response of BALB/c 3T3 cells and program cells to respond to the progression factors required for G0/G1 traverse and entry into S phase.

Original languageEnglish (US)
Pages (from-to)400-407
Number of pages8
JournalMethods in Enzymology
Volume147
Issue numberC
DOIs
StatePublished - Jan 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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