Characterization of Haemophilus ducreyi-specific T-cell lines from lesions of experimentally infected human subjects

V. Gelfanova, T. L. Humphreys, Stanley Spinola

Research output: Contribution to journalArticle

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Abstract

Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted genital ulcer disease that facilitates the transmission of human immunodeficiency virus. In the human model of infection, the histopathology of infected sites in part resembles a delayed-type hypersensitivity (DTH) response. In this study, T cells were isolated from skin biopsy specimens obtained from 24 subjects who were infected for 7 to 14 days. One clone and 12 lines that responded to H. ducreyi antigens were obtained from 12 of the subjects. Fluorescence-activated cell sorter analysis showed that the antigen-responsive lines and clone were predominantly CD3+ and CD4+. The lines and clone responded to H. ducreyi antigen in a dose-dependent manner and produced gamma interferon (IFN-γ) alone or IFN-γ and interleukin-10 (IL-10) but no IL-4 or IL-5 in response to H. ducreyi. Proliferation of T cells was dependent on the presence of autologous antigen-presenting cells. The lines showed little response to antigens prepared from other members of the Pasteurellaceae and responded to different fractions of H. ducreyi separated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We conclude that T cells that recognize H. ducreyi antigens are recruited to sites experimentally infected with the organism. The lack of cross-reactivity to the Pasteurellaceae and the response of the lines to different antigen fractions suggest that subjects are sensitized to H. ducreyi during the course of infection.

Original languageEnglish (US)
Pages (from-to)4224-4231
Number of pages8
JournalInfection and Immunity
Volume69
Issue number7
DOIs
StatePublished - 2001

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Haemophilus ducreyi
Pasteurellaceae
T-Lymphocytes
Cell Line
Clone Cells
Antigens
Chancroid
Interleukin-5
Autoantigens
Delayed Hypersensitivity
Antigen-Presenting Cells
Infection
Sodium Dodecyl Sulfate
Interleukin-4
Interleukin-10
Interferons
Interferon-gamma
Ulcer
Polyacrylamide Gel Electrophoresis
Fluorescence

ASJC Scopus subject areas

  • Immunology

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Characterization of Haemophilus ducreyi-specific T-cell lines from lesions of experimentally infected human subjects. / Gelfanova, V.; Humphreys, T. L.; Spinola, Stanley.

In: Infection and Immunity, Vol. 69, No. 7, 2001, p. 4224-4231.

Research output: Contribution to journalArticle

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